Two multivariate calibration methods are compared for the simultaneous chromatographic determination and separation of Sulfamethoxazole (SMX) and Phthalazine (PHZ) by High Performance Liquid Chromatography (HPLC). Multivariate calibration techniques such as Classical Least Squares (CLS) and Inverse Least Squares (ILS) were introduced into HPLC to determine the quantification by using UV detecto...

This thesis considers issues related to the multivariate analysis and calibration of chemical data collected in a vector format (one–way data). The work is designed to facilitate the task of the analytical chemist, focusing on methods of maximizing the amount of information that can be obtained by multivariate modeling from full sets of data generated by instruments or experiments. It is shown ...

In this paper we propose a robust probabilistic multivariate calibration (RPMC) model in an attempt to identify linear relationships between two sets of observed variables contaminated with outliers. Instead of the Gaussian assumptions that predominate in classical statistical models, RPMC is closely related with the multivariate Student's t-distribution over noises and latent variables. As a r...

attempts to obtain experimental values for the kinetic parameters of phenanthridine oxidation by guinea pig or rabbit liver aldehyde oxidase using common spectrophotometric methods have not been successful due to a lower limit of detection. in the present study, a new spectrofluorimetric assay in combination with a multivariate calibration method for enzymatic kinetic study of aldehyde oxidase ...

Two spectrophotometric methods are described and applied to resolve ternary mixtures of the corticosteroid hydrocortisone (HYD) and the antibiotics nystatin (NYS) and oxytetracycline (OXY). The simultaneous determination of these three compounds was firstly accomplished by a derivative method using the “ratio spectrum-zero crossing derivative” and then by multivariate methods partial least squa...

An analytical methodology based on spectrophotometric and partial least squares (PLS) algorithm for thesimultaneous determination of ampicillin and penicillin in human plasma was developed and validated. Themultivariate model was developed as a binary calibration model and it was built and validated with anindependent set of synthesis and real samples in presence of matrix. It is shown how a de...