Unintentional surgical damage to nerves is mainly due to poor visualization of nerve tissue relative to adjacent structures. Multispectral photoacoustic tomography can provide chemical information with specificity and ultrasonic spatial resolution with centimeter imaging depth, making it a potential tool for noninvasive neural imaging. To implement this label-free imaging approach, a multispect...

Calibrating the elements of a multispectral imaging system. Miguel A. López-Álvarez, Javier Hernández-Andrés, Javier Romero, Joaquín Campos and Alicia Pons. Large Format Printing Division, Hewlett-Packard Spain, 08174 Sant Cugat del Vallès, Barcelona, SPAIN. [email protected] Color Imaging Laboratory, Departamento de Óptica, Universidad de Granada, 18071 Granada, SPAIN. [email protected], jromero@u...

For years, scientists have used thermal broadband cameras to perform target characterization in the longwave (LWIR, 812 m) and midwave (MWIR, 3-5 m) infrared. The analysis of broadband imaging sequences typically provides energy, morphological and/or spatiotemporal information. However, there is very little information about the chemical nature of the investigated targets when using such syst...

We present the development at ENST of a multispectral imaging system. Various methods have been implemented and tested for the characterisation of spectral camera sensitivity, the optimal choice of filters and the reconstruction of the spectral reflectance of the imaged surfaces. We first used a 4k-linear array camera with a set of large band filters. We are now experimenting with a cooled 1k x...

Multispectral fluorescence imaging (MSFI) is a rapidly growing field with broad applications in both preclinical and clinical settings. Application of this novel technology in small-animal imaging and microscopy produces enhanced sensitivity and reliable quantification and resolves multiple simultaneous signals. MSFI flow cytometry can quantify multiple fluorescent parameters with morphologic o...

Our understanding of the molecular mechanisms that direct cell motility, cell division, and cell shaping has benefited from innovations in cell labeling and the ability to resolve intracellular dynamics with multispectral, high-resolution imaging. However, due to difficulties with in vivo cell marking and monitoring, most studies have been restricted to fixed tissue or cells in culture. Here, w...

An application of multispectral imaging in multimedia is presented. We describe a system for the acquisition of multispectral images using a CCD camera with carefully selected optical filters. We further discuss how the surface spectral reflectance of the imaged objects may be reconstructed from the camera response. We then present an application where the acquired multispectral images are used...

Thanks to some technical progress in interferencefilter design based on different technologies, we can finally successfully implement the concept of multispectral filter array-based sensors. This article provides the relevant state-of-the-art for multispectral imaging systems and presents the characteristics of the elements of our multispectral sensor as a case study. The spectral characteristi...

Multispectral imaging is a highly desirable modality for material-based analysis in diverse areas such as food production and processing, satellite-based reconnaissance, and biomedical imaging. Here, we present nanofilter-based multispectral videography (nMSV) in the 700 to 950 nm range made possible by the tunable extraordinary-optical-transmission properties of 3D metallic nanostructures. Mea...