This study aims to produce a mix of enzymes through Solid State Fermentation (SSF) raw materials. Four different, easily available, agro-industrial wastes were evaluated as SSF substrates for production by Cryphonectria parasitica (Murr.) Barr. environmental strains named CpA, CpB2, CpC4, and CpC7. Among the tested wastes, organic wheat bran human use animal feed better supports C. growth prote...

The feasibility of multi-step, one-flask total synthesis of natural products is demonstrated by cloning and overexpression of the corresponding biosynthetic enzymes from plant and microbial sources. As many as eight or nine enzymes can be recombined in vitro to reach an advanced intermediate in the vitamin B12 pathway.

Some metabolic pathway enzymes are known to organize into multi-enzyme complexes for reasons of catalytic efficiency, metabolite channeling, and other advantages of compartmentalization. It has long been an appealing prospect that de novo purine biosynthesis enzymes form such a complex, termed the "purinosome." Early work characterizing these enzymes garnered scarce but encouraging evidence for...

Abstract The inclusion of exogenous digestive enzymes and probiotics is well established in the aquafeed industry. mixture multi-enzymes improves feed utilization wellbeing aquatic animals compared to individual supplementation. Herein, we evaluated multi-enzyme (beta-glucanase, cellulase, alpha-amylase, protease, xylanase, phytase) at 250 mg/kg multi-species probiotic ( Bacillus subtilis , Lac...

Nonribosomal peptide synthetases (NRPSs) are giant multi-domain enzymes that catalyse the biosynthesis of many commercially important peptides produced by bacteria and fungi. Several studies over the last decade have shown that many of the individual domains within NRPSs exhibit significant substrate selectivity, which impacts on our ability to engineer NRPSs to produce new bioactive microbial ...