BACKGROUND This study was conducted to evaluate fibroblast co-culture and Activin A on in vitro maturation and fertilization of mouse preantral follicles. METHODS The ovaries from 12-14-day-old mice were dissected, and 120-150 μm preantral follicles were cultured individually in α-MEM as based medium for 12 days. A total number of 456 follicles were cultured in four conditions: (i) base mediu...

The possibility to reprogram somatic human cells will greatly and deeply change genetic approach and allow the development of new tools to study genetics diseases. Indeed, our ability to study human genetic diseases suffers from the lack of valid in vitro models. The latter should (i) be originating from human primary cells, (ii) be able to self-renew for a long time and (iii) be able to differ...

Single-cell RNA (scRNA) analysis has made it possible for researchers to investigate various biological processes, including development and differentiation. Previous scRNA studies on developing mouse skin have helped shed light the molecular cellular processes involved in regeneration of this tissue. However, these primarily focused either fetal stage or early postnatal period, leaving a gap o...

We have shown previously that phagocytosis of cells dying by apoptosis results in transfer of whole or fragments of chromosomes into the nucleus of the recipient cell. Although DNA transfer was detected in normal cells, stable propagation of the transferred DNA was only observed in cells deficient in p53. Here we show that mouse embryonic fibroblast cells lacking the p21 (Cip1/Waf1) cyclin-kina...

Necroptosis is a well-known form of caspase-independent cell death. Necroptosis can be triggered by various extrinsic stimuli, including death ligands in the presence of receptorinteracting protein kinase 3 (RIPK3), a key mediator of necroptosis induction. Our recent studies have revealed that C-terminus HSC-70 interacting protein (CHIP), an E3 ligase, can function as an inhibitor of necroptosi...

introduction: embryonic stem (es) cells are pluripotent cells that derived from inner cell mass (icm) of blastocyst stage and are able to differentiate in vitro into different cells. purpose: this study was initiated to evaluate the effect of basic fibroblast growth factor (bfgf) on differentiation of es cells into cardiomyocytes among with their response to cardiac drugs. methods: the mouse em...

The Rockefeller University Press $30.00 J. Cell Biol. Vol. 206 No. 3 395–413 www.jcb.org/cgi/doi/10.1083/jcb.201403039 JCB 395 Correspondence to Christopher E. Turner: [email protected] Abbreviations used in this paper: apFRET, acceptor photobleaching FRET; CDM, cell-derived matrix; co-IP, coimmunoprecipitation; FA, focal adhesion; FRET, fluorescence resonance energy transfer; HDAC, histone ...