Under two monoxenic culture techniques of growing plants (filter paper and silica sand cultures), sugar in root exudate from Meloidogyne incognita-infected tomato increased 133 to 836% over controls. In contrast, amino acids were moderately reduced 52 to 56%. Chromatographic analysis showed that galled root exudate contained three sugars, twelve amino acids, and three organic acids, whereas hea...

Monoxenic cultures of Heterodera zeae, the corn cyst nematode (CCN), were established on root explants of corn Zea mays L., cv. Kenworthy. The life cycle of H. zeae was determined from light anti scanning electron microscopic observations of the root explants grown in the dark at 29.5 +/- .5 C under gnotobiotic conditions. The life cycle, from the time the explants were inoculated with second-s...