Brønsted acid sites in zeolites catalyze alkene hydrogenation with H2 via the same kinetically-relevant (C-H-H) + carboniumion-like transition states as those involved in monomolecular alkane dehydrogenation. Reactions between C3H6 and H2 selectively form C3H8 (>80% carbon basis) at high H2/C3H6 ratios (>2500) and temperatures (>700 K). Ratios of C3H8 dehydrogenation to C3H6 hydrogenation rate ...

Recombinant DNA methods are being widely used to express proteins in both prokaryotic and eukaryotic cells for both fundamental and applied research purposes. Expressed protein must be well characterized to be sure that it retains the same properties as the native one, especially when expressed protein will be used in the pharmaceutical field. In this aim, interfacial and kinetic properties of ...

Crystalline bacterial cell surface layers (S-layers) are monomolecular arrays of (glyco)proteins that have recently produced a wealth of new opportunities in nanotechnology. Whereas the in vitro imaging of isolated S-layers is well established, their direct imaging on live cells remains very challenging. Here we use atomic force microscopy (AFM) to visualize S-layer nanoarrays on living Coryneb...

Leaping methods provide for efficient and approximate time stepping of chemical reaction systems modeled by continuous time discrete state stochastic dynamics. We investigate the application of leaping methods for ‘‘small number and stiff’’ systems, i.e. systems whose dynamics involve different time scales and have some molecular species present in very small numbers, specifically in the range ...

A model is proposed for polymer- and salt-induced toroidal condensates of DNA, based on a recent theory of the undulation enhancement of the electrostatic interaction in the bulk hexagonal phase of semiflexible polyions. In a continuum approximation, the thermodynamic potential of a monomolecular toroid may be split up in bulk, surface, and curvature contributions. With the help of an approxima...

The stochastic dynamics of a well-stirred mixture of molecular species interacting through different biochemical reactions can be accurately modelled by the chemical master equation (CME). Research in the biology and scientific computing community has concentrated mostly on the development of numerical techniques to approximate the solution of the CME via many realizations of the associated Mar...

The reduction of ferricytochrome c and ferricytochrome c carboxymethylated at the haem-linked methionine (residue 80) by Cr(2+) ions was studied by stopped-flow techniques. At pH6.2 the kinetics of reduction of ferricytochrome c are simple and correspond to a second-order rate constant of 1.21x10(3)m(-1).s(-1). Under identical conditions the kinetics of reduction of the carboxymethyl derivative...