In this study, a series of molecularly imprinted polymers (MIPs) were synthesised using (1R,2S)-(–)-ephedrine or (1S,2S)-(+)-pseudoephedrine as template, methacrylic acid functional monomer and ethylene glycol dimethacrylate cross-linking agent. A highly selective sensitive method the best performing MIP sorbent in solid-phase extraction (SPE) followed by liquid chromatography-high resolution m...

To test the possibility that a functionally abnormal fibrinogen may exist in some patients with liver disease, we studied the plasma and purified fibrinogens of five patients whose plasma thrombin times were prolonged at least 40% over normal controls. In no patient was there evidence of disseminated intravascular coagulation and/or fibrinolysis. No abnormalities were detected by immunoelectrop...

OBJECTIVES The purpose of this study was to analyze the behavior in water, related to mechanical properties, of experimental composites for dental restoration. METHODS The studied materials were composed of a visible-light-curing monomer mixture (Bis-GMA and TEGDMA or HEMA) and micrometric, nanometric or a mixture of both sizes hydroxyapatite particles as a reinforcing filler. Filler particle...

OBJECTIVES The machinery of peptidoglycan biosynthesis is an ideal site at which to look for novel antimicrobial targets. Phage display was used to develop novel peptide inhibitors for MurC, an essential enzyme involved in the early steps of biosynthesis of peptidoglycan monomer. METHODS We cloned and overexpressed the murA, -B and -C genes from Pseudomonas aeruginosa in the pET expression ve...

The monomer-dimer association constant of solubilized and delipidated sarcoplasmic reticulum ATPase was measured by large zone elution gel chromatography in the presence of a high concentration (18.6 mM) of the nonionic detergent dodecyloctaethylene glycol monoether (C12E8) and of different ATPase protein concentrations in the range of 0.74 (6.4 nM monomers) to 30 (0.26 microM monomers) microgr...

Slow release formulations of the anionic herbicide sulfosulfuron (SFS) were prepared by incorporating it in micelles of an organic cation octadecyltrimethylammonium, which adsorb on the clay-mineral montmorillonite. The fraction of SFS adsorbed on the micelle-clay complex reached 98%, whereas for monomer-clay complexes, its adsorption was insignificant. Fluorescence studies showed surface conta...

Radical ring-opening polymerization (RROP) of cyclic ketene acetals allows for the synthesis functional and biodegradable polyesters. To gain a better understanding RROP, kinetic studies this reaction method are thus essential but still rare. In here we conducted experiments on RROP 2-methylene-1,3,6-trioxocan (MTC) first time. line with earlier findings, behavior could be distributed into chai...

The oligomeric state in solution of four plant annexins, namely Anx23(Ca38), Anx24(Ca32), Anx(Gh1), and Anx(Gh2), was characterized by sedimentation equilibrium analysis and gel filtration. All proteins were expressed and purified as amino-terminal His(n) fusions. Sequencing of the Anx(Gh1) construct revealed distinct differences with the published sequence. Sedimentation equilibrium analysis o...

Introduction Polymethylmethacrylate bone cement has been frequently used as an adjunctive material in the limb salvage surgery for malignant bone tumors. The purpose of this study is to investigate the feasibility of bone cement as a vehicle of methotrexate for local treatment of osteosarcoma, and the cytotoxic effect of the eluted methotrexate on the osteosarcoma cells. Also we evaluated the c...