A multilocus sequence typing (MLST) scheme for Candida krusei was devised, based on sequencing of six gene fragments of the species. The existence of heterozygous results for each of the six fragments sequenced confirms that C. krusei is diploid for at least part of its genome. The C. krusei MLST scheme had a discriminatory index of 0.998, making this system ideal for strain typing of C. krusei...

Inadequate discriminatory power to distinguish between L. pneumophila isolates, especially those belonging to disease-related prevalent sequence types (STs) such as ST1, ST36 and ST47, is an issue of SBT scheme. In this study, we developed a multilocus sequence typing (MLST) scheme based on two non-virulence loci (trpA, cca) and three virulence loci (icmK, lspE, lssD), to genotype 110 L. pneumo...

Staphylococcus chromogenes is a common skin commensal in cattle and has been identified as frequent cause of bovine mastitis intramammary infections. We have developed seven locus Multilocus Sequence Typing (MLST) scheme for typing S . Sequence-based systems, such MLST, application studies genetic diversity, population structure, epidemiology, including strain variation factor pathogenicity or ...

We investigated the genetic relationships of 54 Escherichia coli O103 strains from humans, animals, and meat by molecular typing of housekeeping and virulence genes and by pulsed-field gel electrophoresis (PFGE). Multilocus sequence typing (MLST) of seven housekeeping genes revealed seven profiles, I through VII. MLST profiles I plus III cover 45 Shiga toxin-producing E. coli (STEC) O103:H2 str...

Relatedness between isolates of Streptococcus pneumoniae can be determined from sequences of multiple genes belonging to the core genome (multilocus sequence typing [MLST]), but these do not provide information on gene content that may affect the potential of isolates to cause invasive pneumococcal disease. Gene content data, obtained using microarrays, were gathered for 40 clinical isolates of...

Effective surveillance and management of pathogenic Escherichia coli relies on robust and reproducible typing methods such as multilocus sequence typing (MLST). Typing of E. coli by MLST enables tracking of pathogenic clones that are known to carry virulence factors or spread resistance, such as the globally-prevalent ST131 lineage. Standard MLST for E. coli requires sequencing of seven alleles...

Campylobacter jejuni is an important foodborne pathogen with global distribution. We describe a genotyping study of collection C. (n = 137) isolated from different broiler farms and multiple sites along the processing line in slaughterhouse Argentina during 2011, 2012 2015. The isolates were genotyped using pulsed-field gel electrophoresis (PFGE) multilocus sequence typing (MLST). Based on PFGE...

the growing number of immunocompromised individuals has increased the incidence of infections caused by candida species during the recent decades. typing of c. albicans on the basis of dna sequences at multiple loci has greatly advanced our knowledge about the epidemiology and phylogeny of candidiasis. the aim of this study was to evaluate the diversity, and genetic relationships among c. albic...

background and objectives: some 2 million tons of chicken meat is produced by iran per annum, positioning iran among the top producers in the region. this study aimed to evaluate the molecular epidemiology and genetic characteristics of sal- monella enterica enteritidis in iran. materials and methods: a representative selection of isolates (n=76), initially genotyped by a 7-locus mlva typing sy...

Enterobacter cloacae is an important emerging pathogen, which sometime causes respiratory infection, surgical site infection, urinary infection, sepsis, and outbreaks at neonatal units. We have developed a multilocus sequence typing (MLST) scheme utilizing seven housekeeping genes and evaluated the performance in 101 clinical isolates. The MLST scheme yielded 83 sequence types (ST) including 78...