APC: Antigen-Presenting cells; β2m: Beta2-Microglobulin; BMT: Bone Marrow Transplantation; DSA: Donor-Specific Antibody; ER: Endoplasmic Reticulum; GvHD: Graft-versus-Host Disease; HLA: Human Leukocyte Antigen; HSCT: Hematopoietic Stem Cell Transplantation; IFN: Interferon; ILT2: Ig-like Transcript 2; ILT4: Ig-like Transcript 4; MHC: Major Histocompatibility Complex; MLR: Mixed Lymphocyte React...

Freeze-thaw preparations of banded Rauscher murine leukemia virus markedly suppressed the in vitro cellular-mediated blastogenic response of murine splenic lymphocytes to phytohemagglutinin-P and to allogeneic cells in two-way mixed-leukocyte reaction. Suppression was shown not to be due to cytotoxicity or to virus-mitogen binding. It is suggested that a virion envelope component interferes wit...

Because abnormalities of the immune system may be involved in the pathogenesis of idiopathic congestive cardiomyopathy (ICCM), we studied suppressor cell function in patients with ICCM. Previously described in vitro techniques were modified for optimal demonstration of concanavalin A (con A)-inducible suppressor activity by peripheral blood mononuclear cells (PBM). Baseline responses in the mix...

conclusions systemic arterial leukocyte filtration reduces the intubation time but has no other beneficial effect on the outcome of patients undergoing cabg or valve surgery. results the postoperative intubation time was significantly shorter in patients with leukocyte filters (0.014). there was no significant difference between two groups regarding other outcome relatedvariables. patients and ...

We investigated the effects of mixed leukocyte reaction (MLR), hydrocortisone (HC) and cyclosporine A (CsA) on the expression of leukocyte adhesion molecules on the mesangial (MC) and endothelial cells (EnC). Cell surface enzyme immunoassay showed that INFnu, IL-1beta, or TNF alpha stimulated expression of ICAM-1, or VCAM-1 on MC after 24 hours. Flow cytometric analysis demonstrated that MLR su...

The regenerative and immunomodulatory activity of mesenchymal stromal cells (MSCs) is partially mediated by secreted vesicular factors. Extracellular vesicles (EVs) exocytosed by MSCs are gaining increased attention as prospective non-cellular therapeutics for a variety of diseases. However, the lack of suitable in vitro assays to monitor the therapeutic potential of EVs currently restricts the...

By screening 720 natural compounds in a standard 2-way allogeneic mixed leukocyte reaction assay, we identified a potent immunosuppressive capacity of crassin acetate (CRA), a coral-derived cembrane diterpenoid. CRA efficiently inhibited allogeneic mixed leukocyte reaction as well as antigen-specific activation of CD4 T cells by bone marrow-derived dendritic cells (DCs). With regard to cellular...

Pathological discharge from the genital tract and other signs of discomfort external genitalia are a common cause visit to gynecologist. The these disorders in most cases is vulvovaginal infection, accompanied (vaginitis) or not (vaginosis) by leukocyte reaction. Methods for treating infection predominantly local therapy systemic agents with highly selective antimicrobial activity. Since signif...