Fragments of mitochondrial DNA from Saccharomyces cerevisiae were joined to the Escherichia coli plasmid pBR322 by the poly(dA):poly(dT) tailing method. A total of 347 transformants containing DNA inserts were isolated. The size of the inserted DNA ranges from 0.5 to 5 kilobases. To screen for mitochondrial tRNA genes, we isolated mitochondrial tRNAs, end-labeled them with 32P, and hybridized t...

Abstract Molecular techniques are powerful tools that can address many research problems in insect ecology. Mitochondrial DNA (mtDNA) is a widely used molecular marker. It easy to use and has favorable biological properties, such as near-neutrality, lack of recombination, clock-like evolutionary rate. However, there some issues involved when using mtDNA data population genetics, species delimit...

The rapid diversification of Myotis bats into more than 100 species is one of the most extensive mammalian radiations available for study. Efforts to understand relationships within Myotis have primarily utilized mitochondrial markers and trees inferred from nuclear markers lacked resolution. Our current understanding of relationships within Myotis is therefore biased towards a set of phylogene...

DNA metabarcoding is a promising approach for rapidly surveying biodiversity and is likely to become an important tool for measuring ecosystem responses to environmental change. Metabarcoding markers need sufficient taxonomic coverage to detect groups of interest, sufficient sequence divergence to resolve species, and will ideally indicate relative abundance of taxa present. We characterized zo...

The green seaweeds Ulva australis and U. pertusa were described from southern Australia Japan, respectively. They are conspecific australis, the currently accepted taxon, is native to temperate marine waters in north-eastern Asia, known be introduced overseas into Australasia, Americas Europe. Although genetics of have been investigated elsewhere, along French coasts origins history introductio...

In the present study we confirm the previously reported high frequency of biochemical markers of mitochondrial dysfunction, namely hyperlactacidemia and increased lactate/pyruvate ratio, in a significant fraction of 210 autistic patients. We further examine the involvement of the mitochondrial aspartate/glutamate carrier gene (SLC25A12) in mitochondrial dysfunction associated with autism. We fo...

Cordyceps militaris is a fungus used for developing health food, but knowledge about its intraspecific differentiation is limited due to lack of efficient markers. Herein, we assembled the mitochondrial genomes of eight C. militaris strains and performed a comparative mitochondrial genomic analysis together with three previously reported mitochondrial genomes of the fungus. Sizes of the 11 mito...

The Pennsylvania grotto sculpin is known from just two caves of the Nippenose Valley in central Pennsylvania, USA. They exhibit emergent troglobitic morphological traits and are second northern-most cave adapted fish world. Two mitochondrial ( 16S rRNA D-loop gene) one nuclear S7 ribosomal protein gene intron) both epigean populations were sequenced. For three markers, a large proportion specim...

OBJECTIVE To identify relationships between insulin resistance (IR) and mitochondrial respiration in perinatally HIV-infected youth. DESIGN Case-control study. METHODS Mitochondrial respiration was assessed in perinatally HIV-infected youth in Tanner stages 2-5, 25 youth with IR (IR+) and 50 without IR (IR-) who were enrolled in the Pediatric HIV/AIDS Cohort Study. IR was defined as a homeo...

The nudibranch genus Doto is taxonomically problematic in particular, and some species are described on the notion of strict monophagy. Here we perform delimitation NE Atlantic species, as well placing them phylogenetically, using two markers: mitochondrial COI nuclear H3. We also study morphology including radular ultrastructure review food specificity. Specimens were first divided into potent...