background there is still no suitable mice model that can completely mimic the human fulminant hepatitis, which sets a block for drug effect evaluation and mechanism researching of human fulminant hepatitis. objectives the aim of this study was to establish an animal model able to mimic the main features of human fulminant hepatitis. materials and methods dimethylnitrosamine (dmn) was peritonea...

A closed-tube polymerase chain reaction (PCR) was developed to allow the rapid detection of African swine fever virus (ASFV) DNA. This assay targets the VP72 gene of ASFV and uses the 5'-nuclease assay (TaqMan) system to detect PCR amplicons, avoiding tube opening and potential cross-contamination of post-PCR products. An artificial mimic was engineered with the TaqMan probe site replaced by a ...

The standard methodology for titrating dengue viruses, the plaque assay, is slow, time consuming and relatively expensive. Other methods require machinery that may not be routinely accessible to all researchers, particularly those in developing nations. We therefore sought to develop a rapid, simplified semiquantitative polymerase chain reaction (PCR) methodology based on the use of a template ...

Orolabial and genital herpes are common conditions caused by simplex virus (HSV) that affect many individuals. Although skin findings may mimic other conditions, an HSV infection still be diagnosed clinically. We present a case of polymerase chain reaction (PCR) negative orolabial in young male with high-risk social history. testing was negative, oral clinically the patient adequately treated I...

OBJECTIVE To find out the effect of miR-544a on the invasion of lung cancer cells and to explore the underlying molecular mechanisms. METHODS Micro-ribonucleic acid (miRNA) expression in two different invasive lung cancer cell lines 95C (low invasive ability) and 95D (high invasive ability) was analyzed by miRNA microarray and real-time quantitative polymerase chain reaction (PCR); miR-544a m...

Four different standardization approaches based on a competitive reverse transcription (RT)-PCR assay were compared with a noncompetitive assay based on an external standard curve. Criteria for assessment were accuracy in quantitation, correctness of recovery, sensitivity, dynamic range, reproducibility, throughput, and convenience of sample handling. As a model system, we used the 5'-noncoding...

Abstract The goal of this study was to demonstrate the functions and specific mechanism long non-coding RNA (lncRNA) GNAS-AS1 in lung adenocarcinoma. Levels lncRNA GNAS-AS1, microRNA (miR)-433-3p, Rab3A were assessed by quantitative real-time PCR (qRT-PCR). target-binding sites miR-433-3p, predicted confirmed bioinformatics tool (StarBase) a dual-luciferase reporter system. Cell proliferation a...

Cervical cancer ranks third in incidence worldwide and is the most frequent gynecological developing countries. This study aimed to investigate effect of crocin on resistance HELA/Ara-C cells chemo-agent Ara-C. which was defined as Ara-C-resistant HELA cell line, were treated with crocin. After that, proliferation, apoptosis, cycle assay examined by MTT assay, AO/EB staining, flow cytometry, re...