In the title compound, [Co(C(6)H(5)N(2)O(4))(2)(H(2)O)(2)]·2C(3)H(7)NO, the Co(II) ion lies on an inversion center and adopts a slightly distorted CoN(2)O(4) octa-hedral geometry binding two bidentate chelating 5-carb-oxy-2-methyl-1H-imidazole-4-carboxyl-ate (H(2)MIDA(-)) monoanionic ligands and two axial aqua ligands. In the crystal structure, inter-molecular O-H⋯O hydrogen bonds link neighbor...

Hellerstein, Marc K., and Richard A. Neese. Mass isotopomer distribution analysis at eight years: theoretical, analytic, and experimental considerations. Am. J. Physiol. 276 (Endocrinol. Metab. 39): E1146–E1170, 1999.—Mass isotopomer distribution analysis (MIDA) is a technique for measuring the synthesis of biological polymers. First developed approximately eight years ago, MIDA has been used f...

Objective : to analyze preliminary data on 5-year clinical and radiographic outcomes in patients with early psoriatic arthritis (PsA) observed as part of the Treat target (T2T) strategy. Patients methods . We examined 37 (18 men 19 women) PsA who met CASPAR criteria (2006), received treatment according principles T2T strategy for 24 months. The mean age was 43.3±11.7 years, median (Me) duration...

Abstract A comprehensive study of the rheological characterization aqueous solutions polyethylene oxide (PEO) with molecular mass 4 × 106, 5 and 8 106 g/mol, respectively, named (4 miDA), (5 (8 miDA) was conducted. large batch samples miDA PEO concentrations varying from 0.1% to 3%, representing range dilute very high viscous hydrated gels, were tested. Steady-state shear flow oscillatory measu...

The discovery of enzyme inhibitors relies on synthetic methods that enable rapid and modular construction of small molecules. Heterocyclic fragments designed to maximize enthalpic interactions with their protein targets represent a particularly desirable class of molecules. Here we describe a reagent that enables straightforward construction of "borofragments", in which a heterocycle is separat...

The measurement of protein kinetics by isotopic techniques has been hindered by the long-standing difficulty of accurately measuring the isotope content of the biosynthetic precursor pool (aminoacyl-tRNA in tissues). Mass isotopomer distribution analysis (MIDA) is a stable isotope-mass spectrometric (MS) technique for measuring biosynthetic precursor enrichments from measurements on a polymeric...

A new stable isotope procedure has been developed and validated in rats, applying [1-(13)C]acetate infusion to quantify the production of bile salts from de novo synthesized cholesterol making use of the mass isotopomer distribution analysis (MIDA) principle. Ions (m/z) 458-461, 370-373 and 285-288 were monitored by GC/MS (EI-mode) for the methyl trimethylsilylether derivatives of cholate, chen...

To quantify the contribution of newly synthesized cholesterol to total plasma and biliary cholesterol under physiological conditions, unrestrained rats were infused intravenously with [1-13C]acetate (0. 6mmol/h per kg) from 12:00 to 24:00 h, and fractional and absolute cholesterol-synthesis rates were determined by mass isotopomer distribution analysis (MIDA). As bile diversion leads to changes...