The enzyme-linked immunosorbent assay (ELISA) has proved to be a sensitive and specific quantitative procedure for the serodiagnosis of toxoplasmosis. Using the toxoplasma model, several parameters of the test were investigated. Day-to-day reproducibility was 90% within one twofold dilution and 98% specific when tested against batteries of sera from other diseases. Both the tube method and the ...

Mobile communication systems are undoubtedly an environmental source of electromagnetic radiation (EMR). Although the direct health effect a cell phone to human is still elusive, unicellular organisms rather apparent. The aim this study was examine effects EMR on development bacterial biofilm. Microtitration plates with four strains Staphylococcus aureus were exposed field frequencies 1-5 GHz, ...

In both the complement fixation test (CFT) and the serum agglutination test (SAT) titres were reproducible for the most part within a twofold range. They seldom exceeded these limits and never a fourfold range. Brucella abortus was successfully isolated in 86% of serologically positive cases and evidence is presented to confirm the use of the 30 International Units/ml level in the CFT as being ...

A modified haemagglutination inhibition test for rubella antibodies using prestandardized freeze-dried reagents was compared to a "standard" method. Tests of 707 serum samples showed that the modified test was sensitive and reliable by both macrotitration and microtitration techniques. The minor disadvantages of some reduction in antibody level when rubella sera were tested within one week of t...

Monoclonal antibody against light chains of human cardiac myosin (MLC) was labelled with horseradish peroxidase. The conjugation was performed by two different methods with glutaraldehyde and periodate respectively. The binding activities of the conjugates were tested by enzyme linked immunosorbent assay (ELISA) on the microtitration plates with immobilized MLC (1-1000 ng per well). A compariso...

Tetanus antitoxin in human sera was detected with solid-phase immunoassays in microtitration modules coated with tetanus toxoid by using Eu+ -labeled anti-human monoclonal antibodies on the basis of an exactly calibrated antibody standard. The use of a time-resolved fluorescence immunoassay (TR-FIA) significantly improved the quantitative detection of tetanus antitoxin over that of the enzyme-l...