Abstract Diverse processes leading to doubled haploid (DH) plant production, such as microspore embryogenesis, gynogenesis, and distant hybridization followed by genome elimination, are based on the unique ability of cells form embryos without fertilization. All these possible because various in vitro culture systems that enable growth development tissues or single outside parental organism. Th...

A simple procedure is described for the mechanical isolation of protoplasts of unfertilized and fertilized barley egg cells from dissected ovules. Viable protoplasts were isolated from ~75% of the dissected ovules. Unfertilized protoplasts did not divide, whereas almost all fertilized protoplasts developed into microcalli. These degenerated when grown in medium only. When cocultivated with barl...

Doubled haploid (DH) technology is a powerful tool in plant breeding to reduce the time and costs needed to produce pure lines, the cornerstone of hybrid seed production. This biotechnological alternative to classic methods allows for a reduction of the typical 7–8 inbreeding generations needed to fix a hybrid genotype to only one in vitro generation. It is therefore much faster and cheaper, be...

Plant growth regulator (PGR) crosstalk and interaction with the plant's genotype and environmental factors play a crucial role in microspore embryogenesis (ME), controlling microspore-derived embryo differentiation and development as well as haploid/doubled haploid plant regeneration. The complexity of the PGR network which could exist at the level of biosynthesis, distribution, gene expression...

Abstract The endogenous level of cytokinins (CKs) and auxins (Auxs) was analyzed in isolated microspores ovaries two doubled haploid (DH) lines triticale (× Triticosecale Wittm.) to better understand the mechanism microspore embryogenesis (ME)—the most efficient widely applied method producing DHs. responsiveness studied ME significantly varied. induced by pre-treating tillers with low temperat...

Ultrastructural features of embryogenic pollen in Datura innoxia are described, just prior to, during, and after completion of the first division of the presumptive vegetative cell. In anther cultures initiated towards the end of the microspore phase and incubated at 28 degrees C in darkness, the spores divide within 24 h and show features consistent with those of dividing spores in vivo. Cytok...

The objective of this study was to produce durum wheat doubled haploid plants through the induction of microspore embryogenesis. The microspore culture technique was improved to maximize production of green plants per spike using three commercial cultivars. Studies on factors such as induction media composition, induction media support and the stage and growth of donor plants were carried out i...

Abstract In eggplant microspore embryogenesis, embryos are produced and then transformed into undifferentiated calli, instead of developing as true embryos. This is the main current bottleneck that precludes this process from being efficient. work we aimed to shed light on factors involved in successful vitro development haploid by evaluating role growth regulators (GRs) process. We analyzed en...

Brassica napus is a species of high agronomic interest, used as model to study different processes, including microspore embryogenesis. The DH4079 and DH12075 lines show low embryogenic response, respectively, which makes them ideal the basic mechanisms controlling embryogenesis induction. Therefore, availability protocols for genetic transformation these two backgrounds would help generate too...

[This corrects the article on p. 424 in vol. 6, PMID: 26113852.].