History of Plant Tissue Culture and Biotechnology Scope and Importance of Biotechnology Tissue and Cell Culture Micropropagation Anther Culture Production of Secondary Metabolites Protoplasts Isolation and Fusion Somatic Hybrids Somaclonal Variation Germplasm Conservation Genetic Engineering and Gene Technology Methods for Gene Transfer Generation of Transgenic Plants and their identification M...

Wadatsuminoki (Nothapodytes amamianus) is an endangered tree species observed in only Amami Oshima Island located in southern part of Japan. According to the Red list published by Ministry of Environment, it is classified as 1A (Critically endangered) and naturally remaining number is only 20. It contains camptotesin which is used for anti-cancer drugs. Kagikazura (Uncaria rhynchophylla) is an ...

Asparagus racemosus (Shatavari) is a plant with immense medicinal values. It is reported to be recalcitrant to tissue culture practices. Sterile cultures were obtained when the explants were treated with (0.5%) Bavestine, Indophyl and 8HQC for 7 min and HgCl2 for 5 min.The present study on micropropagation of Asparagus racemosus through nodal explants showed better result in BAP (2 mg/l) along ...

Different in vitro culture techniques have been used for rapid plant propagation. Pathogenfree plants have been produced using techniques such as seed culture, meristem culture, micropropagation using axillary or adventitious shoot buds, and somatic embryogenesis. This Bulletin discusses the importance of in vitro culture techniques for the production of pathogen-free medicinal plants such as A...

An efficient clonal propagation procedure for six rice varieties cultivated in Argentina was developed by using shoot tip cultures, and the genetic stability of the micropropagated plants was verified by isozyme analysis. One week old seedlings obtained on MS medium were sectioned and subcultured on MS medium (0.75% agar) supplemented with different combination and concentrations of cytokinins ...

Numerous workers have shown that vegetative propagation of eucalypt was possible at the industrial level using physiologically and onto genetically mature plants. This kind of propagation needs the use of rejuvenated shoots which can be obtained by intense pruning, grafting and successive graftings (with a great attention to take cutting or graft on the precedent generation as soon as possible)...

Seeds of Bauhinia acuminata L. were used as an experimental material for micropropagation. The effect of growth regulators concentrations on morphogenetic development was studied. Seeds were germinated on MS medium supplemented with GA3 (0.10, 1.0 and 2.0 mgL -1 ) and without GA3. Satisfactory germination was observed at media with 1.0 mgL -l GA3. Subsequent propagation from plantlet was perfor...