This study was designed to investigate a previously unidentified potential mechanism for mutation induction as well as to clarify a biological consequence of micronuclei with mutation induction as measured by trifluorothymidine (TFT) resistance in mouse L5178Y cells using four aneugens: colcemid, diethylstilbestrol, griseofulvin and vinblastine. All four compounds induced micronuclei which appe...

Objective: Nanotechnology is an emerging technology which has wide applications in many fields. Prime concern of research nanotechnology the synthesis nano-material with controlled size and shape. Recently, biosynthesis metallic nano-particles gained popularity owing to its eco-friendliness. The increasing use Copper nanoparticles (CuNPs) medicine industry demands understanding their potential ...

Loss of heterozygosity is one genetic change observed in many tumours. We do not know whether the loss of chromosomal material through micronucleus formation is a viable mechanism associated with, and possibly leading to, genetic disease. Previously, we treated L5178Y mouse lymphoma cells with four aneugens. Although these aneugens induced micronuclei containing predominantly whole chromosomes,...

Micronuclei in exfoliated epithelial cells are widely used as biomarkers of cancer risk in humans. To elucidate the effect of different staining procedures on the outcome of such investigation, we conducted a study in which the micronuclei frequencies in oral mucosa cells of heavy smokers (n = 20) and nonsmokers (n = 10) were evaluated with nonspecific (Giemsa, May-Grünwald-Giemsa) and DNA-spec...

A micronucleus is a small nucleus-like structure found in the cytoplasm of dividing cells that suffered from genotoxic stress. It is generally hypothesised that micronuclei content is eventually lost from cells, though the mechanism of how this occurs is unknown. If DNA located within the micronucleus is not replicated, it may explain the loss of micronuclei content. Because there had been no c...

The primary aim of this study was to quantify genetic and environmental influences on the frequency of spontaneously occurring micronuclei in children and adults. To meet this aim, a total of 63 male and female twin pairs and 19 singletons (145 individuals) were evaluated, ranging in age from 7 to 85 years. Micronuclei frequencies significantly increased with age for both genders (r = 0.49, P <...

background: in many studies, chemicals and natural materials were tested to reduce the harmful effects of radiation. it is known that famotidine and vitamin c reduce dna damage. objective: the aim of this study was to evaluate the radioprotective effect of vitamin c, cimetidine and famotidine on gamma-radiation-induced damage on mouse bone marrow.  methods: six-to-seven week male nmri mice (28 ...

The genotoxic effects of 4 organophosphorous pesticides, i.e. ekatin, fenitrothion, methylparathion and phorate, were examined employing the micronucleus test in bone marrow cells of the rat. Methylparathion and phorate were found to be mutagenic, while ekatin was weakly mutagenic. The frequency of micronuclei induction by fenitrothion did not differ significantly from that noticed in negative ...

BACKGROUND Ionizing radiation is a well-known carcinogen. Chromosome aberrations, and in particular micronuclei represent an early biological predictor of cancer risk. There are well-documented associations of micronuclei with ionizing radiation dose in some radiation-exposed groups, although not all. That associations are not seen in all radiation-exposed groups may be because cells with micro...