In the reference laboratory complete serological identification of salmonella requires the identification of 59 'O' (somatic) and 87 'H' (flagellar) antigens. When large numbers of strains are studied mechanisation of the serological techniques would be advantageous. Normally, slide and tube agglutination methods are used,' 2 and the latter is an essential confirmatory test. The antisera used a...

A significantly greater incidence (P less than 0.005) of Legionella pneumophila microagglutination titers greater than or equal to 32 was found in sera with elevated titers to Pseudomonas pseudomallei as compared with sera with negative titers for P. pseudomallei antibodies. The greater incidence of L. pneumophila titers in these sera suggests that L. pneumophila and P. pseudomallei share an an...

A quantitative microagglutination test for measuring group A streptococcal antibody levels is described and evaluated. The pro­ cedure requires only a patient’s serum and a standardized antigen consisting of pepsin and trypsin treated group A streptococcal cells. The antibody measured appears to be directed mainly at the group A carbohydrate of the cell wall. In “normal” populations, basal tite...

Leptospirosis is a zoonosis with protean clinical manifestations. Its diagnosis requires a high index of suspicion and is confirmed by isolation of the organism or, more commonly, by serologic tests. In the fall of 1987, after severe flooding, we saw 93 patients with leptospirosis, confirmed by a microagglutination test. Thirteen percent of the patients had no clinical or laboratory findings ex...

OBJECTIVES Brucellosis is one of the most common zoonoses in the world, and occurs mainly in farmers, slaughterhouse workers, and veterinarians via direct or indirect contact with infected animals or their products. The clinical symptoms of human brucellosis are nonspecific, such as fever, headache, chills, and sweating. Diagnosis and treatment of brucellosis requires laboratory tests. Although...

Swine dysentery is a mucohemorrhagic diarrheal disease caused by the anaerobic spirochete Serpulina hyodysenteriae. At present, the serotyping is done by immunodiffusion testing with lipopolysaccharide (LPS) extract as antigen and rabbit hyperimmune sera produced against different serotypes of S. hyodysenteriae. Since the preparation of LPS is time-consuming and requires a large quantity of bac...

Little is known about host immunodynamics during Brucellosis. We conducted immunohematological study in Brucellosis affected cattle for 3 months. Serum proteins and globulins were decreased very significantly (P<0.01) from zero day (0D) to 90D and significantly (P<0.05) from 0D to 30D. Globulins decreased significantly (P<0.05) from 0D to 75D. Titers of anti-Brucella antibodies by Standard Tube...