THE reduction of methylene blue by lactic acid, or in other words, the dehydrogenation of lactic acid in the presence of extracts from animal tissues, is now known to be catalysed by a system composed of two enzymes (lactic dehydrogenase and diaphorase flavoprotein) and cozymase [Dewan & Green, 1938; Euler & Hellstrom, 1938]. The chemical reactions involved in this process may be written [Corra...

The removal of Methylene Blue dye from aqueous solution using a biowaste, dried Blue Green Algae was investigated in terms of variation of initial concentration of the dye solution, adsorbent dosage and pH. The optimum pH for the removal of dye by adsorption was found to be pH 7. The data obtained in this study fitted with Langmuir and Freundlich adsorption isotherms. The experimental data conf...

Novel DNA-sensor with enzymatic amplification of the signal has been developed on the base of glassy carbon electrode modified with ds-DNA and horseradish peroxidase (HRP). Phenothiazine dyes Methylene Blue and Methylene Green were used as electrochemical markers for the detection of sulfonamide and anthracycline preparations able to interact with DNA. The biosensor signal related to HRP oxidat...

A simplistic green and neat procedure was developed for the synthesis of arylmethyl and heteroaryl phosphonates or phosphinates by silica gel supported Lewis acid catalyst, CeCl3.7H2O-SiO2 through Michaelis-Arbuzov reaction under conventional as well as microwave irradiation methods. Herein, tetraalkyl 1,4phenylenebis(methylene)diphosphonates 5(a-d)/dimethyl 1,4-phenylenebis(methylene)bis (phen...

Oxidized indophenols and methylene blue are bacteriostatic for Pneumococcus and hemolytic streptococci of human and bovine origin, while the indigoes, malachite green and litmus are not toxic. 2-Chloroindophenol, the most positive of the indicators of oxidation-reduction potentials used, is also the only one to have a bacteriostatic action on cheese strains of Streptococcus haemolyticus. Methyl...

A soluble, cytochrome linked flavoprotein which oxidizes lactic acid to pyruvic acid has been isolated from Penicillium chrysogenum, strain NRRL 1951 -B25. The reduction of cytochrome c by a flavin enzyme has precedence in the work of Horecker and Heppel (1949) and Mlorell (1952) who have shown that xanthine oxidase can reduce cytochrome c. Morell reported the enzyme to be inhibited strongly by...

A 61-year-old alcoholic man presented to the emergency department because of vomiting and dyspnea after discharged from hospital due to appendectomy two days ago. He smoked heavily and his past medical history revealed gastritis. On the examination, he was hypotension (50/00 mmHg) and lethargy. Cardiopulmonary resuscitation was initiated aggressively. His chest x-ray showed right-sided tension ...

Synthesis of zeolite from kaolinite clay is a very promising process, requiring thorough beneficiation and calcinations to obtain purer monomer. Kaolinite clay sourced from Kankara, Nigeria is associated with inherent impurities, making its transformation rather challenging. The addition of dye, though, assisted in inhibiting the negative effects of impure phases on the desired end products and...

The technique of low energy (0-30 eV) electron impact spectroscopy, originally developed for gas phase molecules, is applied to room temperature ionic liquids (IL). Electron energy loss (EEL) spectra recorded near threshold, by collecting 0-2 eV electrons, are largely continuous, assigned to excitation of a quasi-continuum of high overtones and combination vibrations of low-frequency modes. EEL...