Background: Methotrexate is an anti-metabolite drug and one of the folic acid analog that it can play an important and functional role in the treatment of many malignancies and inflammatory diseases. However, the development of petechiae (maculopapular rash) is a very rare complication. Case Presentation: The patient was a 77 years old woman, Aryan race, who was known a case of severe and prog...

Methotrexate reduces intracellular pools of 5-methyltetrahydrofolate and could result in reduced conversion of homocysteine to methionine by methionine synthetase. This study was designed to investigate the effects of moderate dose to very high dose methotrexate on methionine and total homocysteine as reflections of methotrexate induced intracellular events. Methionine and total homocysteine we...

Three techniques for measuring methotrexate show various cross reactivities with methotrexate-related compounds: "high-pressure" liquid chromatography, by principle, is virtually specific for methotrexate; the enzyme-inhibition assay quantitates methotrexate, methotrexate diglutamate, and methotrexate triglutamate equally well, but has a 10% cross reactivity with 4-amino-4-deoxy-N10-methylptero...

The efflux of methotrexate and methotrexate polyglutamates was examined with primary monolayer cultures of rat hepatocytes and also with monolayer cultures of a stable hepatocarcinoma cell line (H35 cells). In this study, the polyglutamates were considered as those species with two or more additional glutamate residues. When methotrexate polyglutamates were present in hepatocytes in excess of m...

OBJECTIVE Clinical trials have not consistently demonstrated differences between tumour necrosis factor inhibitor (TNFi) plus methotrexate and triple therapy (methotrexate plus hydroxychloroquine plus sulfasalazine) in rheumatoid arthritis (RA). The study objective was to estimate the efficacy, radiographic benefits, safety and patient-reported outcomes of TNFi-methotrexate versus triple therap...

a study was undertaken to evaluate the nucleolar organizing regions (nor) count and histopathological changes in rat’s liver treated with methotrexate (mtx). male wistar rats aged 4 months, maintained in our institution were used in the present study. animals were injected intraperitonealy with 3 different doses of methotrexate on alternative days for one week and the animals were sacrificed on...

OBJECTIVE To compare methotrexate based disease modifying antirheumatic drug (DMARD) treatments for rheumatoid arthritis in patients naive to or with an inadequate response to methotrexate. DESIGN Systematic review and Bayesian random effects network meta-analysis of trials assessing methotrexate used alone or in combination with other conventional synthetic DMARDs, biologic drugs, or tofacit...

OBJECTIVE To test the hypothesis that intravenous use of methotrexate associated with lipid nanoemulsions can achieve superior anti-inflammatory effects in the joints of rabbits with antigen-induced arthritis compared with commercial methotrexate. METHODS Arthritis was induced in New Zealand rabbits sensitized with methylated bovine serum albumin and subsequently intra-articularly injected wi...

       This study was designed to explore the protective effects of ascorbic acid and water extract of Spirulina platensis (blue green algae) on methotrexate-induced lipid peroxidation using goat liver as the lipid source. This in vitro evaluation was done by measuring the malondialdehyde, 4-hydroxy-2-nonenal, reduced glutathione and nitric oxide content of tissue homogenates....

methotrexate is an anti-rheumatic agent used as a first-line tretment for rheumatoid arthritis. hematological malignancies like lymphoma or acute myeloid leukemia have been reported to be secondary to treatment with methotrexate, but are very uncommon. we report here the first moroccan case of ra patient on low dose mtx, who developed aml. we also reviewed all similar cases.