نتایج جستجو برای: metaphase

تعداد نتایج: 5816  

Journal: :Current Biology 2009
Sung Hugh Choi Marie-Pierre Péli-Gulli Iain Mcleod Ali Sarkeshik John R. Yates Viesturs Simanis Dannel McCollum

BACKGROUND It is unknown how oscillations in Cdk1 activity drive the dramatic changes in chromosome and spindle dynamics that occur at the metaphase/anaphase transition. RESULTS We show that the Schizosaccharomyces pombe monopolin complex has distinct functions in metaphase and anaphase that are determined by the phosphorylation state of its Mde4 subunit. When Cdk1 activity is high in metapha...

Journal: :Micromachines 2011
Pranjul Shah Indumathi Vedarethinam Dorota Kwasny Lars Andresen Søren Skov Asli Silahtaroglu Zeynep Tümer Maria Dimaki Winnie E. Svendsen

We present a novel integrated device for preparing metaphase chromosomes spread slides (FISHprep). The quality of cytogenetic analysis from patient samples greatly relies on the efficiency of sample pre-treatment and/or slide preparation. In cytogenetic slide preparation, cell cultures are routinely used to process samples (for culture, arrest and fixation of cells) and/or to expand limited amo...

Journal: :Journal of cell science 1978
P M Wassarman K Fujiwara

Immunofluorescent anti-tubulin staining has been used to follow nuclear progression from dictyate to metaphase II during meiotic maturation of mouse oocytes in vitro. Antibody directed against tubulin isolated from sea-urchin eggs decorates the metaphase I and metaphase II spindles, as well as the cytoplasmic bridge, midbody, and polar body of the maturing mouse oocytes. Changes in the tubulin-...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1995
M Hershkovitz A D Riggs

We report that ligation-mediated PCR (LMPCR) can be used for high-resolution study of metaphase chromosomes, and we discuss the role of metaphase chromatin structure in the preservation of differentiated cell states. The X chromosome-linked human PGK1 (phosphoglycerate kinase 1) promoter region was investigated, and euchromatic active X chromosome (Xa) metaphase chromatin was compared with inte...

Journal: :Development 1991
T Choi F Aoki M Mori M Yamashita Y Nagahama K Kohmoto

p34cdc2 protein kinase is a universal regulator of M-phase in eukaryotic cell cycle. To investigate the regulation of meiotic and mitotic cell cycle in mammals, we examined the changes in phosphorylation states of p34cdc2 and its histone H1 kinase activity in mouse oocytes and embryos. We showed that p34cdc2 has three different migrating bands (referred to as upper, middle and lower bands) on S...

Journal: :Development 1988
H J Clarke J Rossant Y Masui

Mouse oocytes at metaphase I were treated with puromycin, which caused the chromosomes to become decondensed within an interphase nucleus. When the oocytes were allowed to resume protein synthesis, they returned to metaphase within 8-10 h and neither synthesized DNA nor cleaved, indicating that they had not been parthenogenetically activated by the puromycin treatment. However, when dibutyryl c...

Journal: :Head & neck 2007
Bernhard Schick Silke Wemmert Ulrike Bechtel Piero Nicolai Thiemo Hofmann Wieslaw Golabek Steffi Urbschat

BACKGROUND Frequent beta-catenin mutations have been detected in juvenile angiofibromas, but the tumor pathogenesis remains unknown. METHODS Metaphase-comparative genomic hybridization (CGH) was used to identify chromosomal aberrations in 29 tumor specimens. Two tumors were investigated using genome DNA microarrays. RESULTS Three hundred eleven chromosomal gains and losses were detected by ...

2016
Kuan-Chung Su Zachary Barry Nina Schweizer Helder Maiato Mark Bathe Iain McPherson Cheeseman

To achieve chromosome segregation during mitosis, sister chromatids must undergo a dramatic change in their behavior to switch from balanced oscillations at the metaphase plate to directed poleward motion during anaphase. However, the factors that alter chromosome behavior at the metaphase-to-anaphase transition remain incompletely understood. Here, we perform time-lapse imaging to analyze anap...

Journal: :Journal of Biological Chemistry 2005

Journal: :Blood 1992
R B Jenkins M M Le Beau W J Kraker T J Borell P G Stalboerger E M Davis L Penland A Fernald R Espinosa D J Schaid

Trisomy 8 is a common anomaly in bone marrow (BM) cells of patients with myeloproliferative disorders (MPD), myelodysplastic syndromes (MDS), or acute nonlymphocytic leukemia (ANLL). We studied the efficacy of fluorescence in situ hybridization (FISH) detection of trisomy 8 in patients with MPD, MDS, or ANLL using directly labeled fluorescent alpha-satellite and whole chromosome paint (WCP) DNA...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید