Ascorbate, 2-mercaptoethanol, and 2,3-dimercaptopropanol (l-3) are used as protective agents to stabilize solutions of tetrahydrofolate and its derivatives. The protective action of these compounds has been thought to be due exclusively to their reducing properties. The spontaneous decomposition of HI-folate to the diazotizable amine, p-aminobenzoylglutamate, has been used to measure quantitati...

introduction: the purpose of this study was to evaluated the effect of β-mercaptoethanol on resumption of meiosis, in vitro maturation of immature mouse oocytes and resulting embryo development with and without bso (dl-buthionine sulfoximine). material and methods: germinal vasicle (gv) were recovered from 6-8 weeks old nmri ovaries and cultured in maturation medium in memα supplemented with 7....

background: brucellosis is a febrile zoonotic infectionand has worldwide distribution among humans as well asanimals. although the seroprevalence of brucellosis in variousanimals has been described in iran, there is only one report onequine brucellosis in the region. objectives: this study wascarried out to determine the seroprevalence of brucellosis inracing clubs and private horse owners in t...

The aim of this study was to evaluate the effects of the addition of antioxidants (vitamin E, β- mercaptoethanol and their combination) to semen extender.  Progressive motility, viability and pH of fresh ram semen were examined. Ejaculate samples were collected with artificial vagina from three Ghezel-Merinose rams (3 years old) during non- breeding reproduction season. Semen was diluted with a...