Background: ROS production is a well-established phenomenon during sperm cryopreservation. Therefore, supplementation of freezing media with antioxidant is an inevitable event. The aim of this study was to assess whether a cell permeable superoxide dismutase agent such as MnTE, can further improve the quality of frozen/ thawed semen sample using a commercially optimized sperm cryopreservation m...

objective: envenomation by heamotoxic snakes constituted a critical health occurrence in the world. bleeding is the most sever consequence following snake bite with viperid and crothalid snakes. it is believed that the degradation of vascular membrane caused hemorrhage; in contrast, some suggested that direct cytotoxicity has role in endothelial cell disturbances. this study was to evaluate the...

This study evaluated the plasma membrane integrity, acrosomal membrane integrity, and mitochondrial membrane potential of Nelore bull sperm from early puberty to early sexual maturity and their associations with sperm motility and vigor, the mass motility of the spermatozoa (wave motion), scrotal circumference, and testosterone. Sixty Nelore bulls aged 18 to 19 months were divided into four lot...

Thioridazine (TZ) is used mainly in the treatment of schizophrenia. However, hepatotoxicity as a life-threatening adverse effect is associated with its clinical use. In this context, we examined the cytotoxic mechanisms of TZ on freshly isolated rat hepatocytes to better understanding of the pathogenesis of TZ-induced hepatotoxicity. Hepatocytes were prepared by the method of collagenase enzyme...

Background: Semen cryopreservation is the most important section of artificial insemination programs; it allows preservation of semen fertility for a long time. Materials and Methods: The aim of the present study was to detect the effect of in vitro supplementation of 4-hydroxy Tempo on fresh and frozen spermatozoa quality of buffalo bulls. Five healthy buffalo bulls (5 ejaculates from each bul...

B acteria commonly encode virulence determinants that mediate the disruption of host cell membranes; however, vacuole lysis and invasion of the host cell cytosol is a strategy used by only a select few bacterial pathogens for intracellular replication (1). Many bacterial pathogens replicate in specialized vacuoles that must be actively maintained to prevent these organelles from fusing with lys...

Objective: Envenomation by heamotoxic snakes constituted a critical health occurrence in the world. Bleeding is the most sever consequence following snake bite with viperid and crothalid snakes. It is believed that the degradation of vascular membrane caused hemorrhage; in contrast, some suggested that direct cytotoxicity has role in endothelial cell disturbances. This study was to evaluate the...

Background: Exposure of sperm membranes to Reactive Oxygen Species induces lipid peroxidation causing membrane breakdown and loss of function. A rosemary extract as dietary additive was assayed for antioxidant effectiveness to improve the quality of rooster semen. Although rosemary extract has been evaluated for in vitro study of semen preservation, up to date no study has been conducted to exa...

BACKGROUND Construction of microbial biocatalysts for the production of biorenewables at economically viable yields and titers is frequently hampered by product toxicity. Membrane damage is often deemed as the principal mechanism of this toxicity, particularly in regards to decreased membrane integrity. Previous studies have attempted to engineer the membrane with the goal of increasing membran...

Thioridazine (TZ) is used mainly in the treatment of schizophrenia. However, hepatotoxicity as a life-threatening adverse effect is associated with its clinical use. In this context, we examined the cytotoxic mechanisms of TZ on freshly isolated rat hepatocytes to better understanding of the pathogenesis of TZ-induced hepatotoxicity. Hepatocytes were prepared by the method of collagenase enzyme...