tumor chromoprotein antibiotics for liver cancer, stomach cancer, bladder cancer, acute leukemia, isolated from culture filtrates of Streptomyces carzinostaticus var. F-41 (S. carzinostaticus). NCS is composed of two components, and acidic single chain polypeptide (apo-NCS) and a labile, ninemembered ring enediyne-containing chromophore (NCSchr). Because NCS has several problems in clinical cas...

Here we describe a simple and cost-effective PCR-based method to introduce deletions, point mutations, frame-shift, or truncations with very high efficiency. The scope and additional applications of the method are discussed. Site-directed mutagenesis involves the introduction of mutations at the DNA level to alter the primary amino acid sequence of proteins (1,2). To approach the desired level ...

Denaturing HPLC (DHPLC) is a relatively novel technique that has been mostly applied to molecular detection of pathological gene alterations (1,2). The method relies on the identification of heteroduplex molecules, due to mismatches between wild-type and mutant alleles, that are visualized in the form of altered chromatograms. Other nonconventional applications of DHPLC have also been reported,...

Site-directed mutagenesis is a widely used technique in molecular biology to generate single or multiple exchanges, insertions or deletions in DNA sequences. A number of strategies have been developed and used successfully (2,4). The QuikChange Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA, USA) has proven valuable because it consistently yields high mutagenesis efficiencies and uses...