The hermaphrodite Pacific oyster Crassostrea gigas displays a high energy allocation to reproduction. We studied the expression of AMP-activated protein kinase (AMPK) during gametogenesis in the gonad and characterized the mRNA sequences of the AMPK subunits: the AMPK alpha mRNA sequence was previously characterized; we identified AMPK beta, AMPK gamma, and mRNAs of putative AMPK-related target...

The effects of aluminum on plasma ion, lipid, protein and steroid hormone concentration were evaluated in Oreochromis niloticus broodstock females. Lipid and protein concentrations from the gonads and liver were also measured. Experiments were performed at neutral and acidic water pH. Four groups of fish were tested for 96h: 1) control conditions at neutral water pH; 2) control conditions at ac...

The following article presents results of the complex research on postulation immunohistochemical features ovaries’ structure in case fetuses with a gestational term 21-28 weeks from mothers HILGT comparing to ones physiological pregnancy. materials are: 25 as well All had died antenatally and inranatally, result violation uteroplacental umbilical-uterine circulation. Research methods: macrosco...

a total of 48 female spirlins alburnoides sp. from the kesselian stream (south caspian sea basin, northern iran) were examined during a period from july 2008 to june 2009 to obtain data on fecundity, and to determine the spawning season of this carp. the mean absolute fecundity of females spirlin was 1722.92 (653.88) eggs. the fecundity has positive correlation with the size of the female. the...

reproductive parameters evaluation is considered as helpful tool for gene bank formation in ecological and economically important animals species. gloydius halys caucasicus is venomous, viviparous pit viper of northwest of iran. in this research, the spermatic reproductive parameters of this taxon were studied. twenty six male snakes were collected from takht-e-soleiman region between september...

Chemical pollution by pesticides has been identified as a possible contributing factor to the massive mortality outbreaks observed in Crassostrea gigas for several years. A previous study demonstrated the vertical transmission of DNA damage by subjecting oyster genitors to the herbicide diuron at environmental concentrations during gametogenesis. This trans-generational effect occurs through da...

The role of miRNA in fish sexual development is not elucidated yet. We profiled miRNAs in gonads and brains of Atlantic halibut using SOLiD sequencing technology. We found tissue- and sexually dimorphic expression of several miRNAs, including miR-29a, miR-34, miR-143, miR-145, miR-202-3p, miR-451, and miR-2188. miR-9 and miR-202 were abundant in brain and gonads, respectively. In the next step,...

Recent examinations of newly obtained materials of dracunculoid nematodes (Dracunculoidea) parasitizing marine fishes off New Caledonia, South Pacific, revealed the presence of several nematodes of the genera Philometra Costa, 1845 (Philometridae) and Ichthyofilaria Yamaguti, 1935 (Guyanemidae), including the following four new species: Philometra priacanthi sp. n. (males) from the gonads of Pr...

The molecular genetic mechanisms of sex determination are not known for most vertebrates, including zebrafish. We identified a mutation in the zebrafish fancl gene that causes homozygous mutants to develop as fertile males due to female-to-male sex reversal. Fancl is a member of the Fanconi Anemia/BRCA DNA repair pathway. Experiments showed that zebrafish fancl was expressed in developing germ ...

The transplantation of germ cells into adult recipient gonads is a tool with wide applications in animal breeding and conservation of valuable and/or endangered species; it also provides a means for basic studies involving germ cell (GC) proliferation and differentiation. Here we describe the establishment of a working model for xenogeneic germ cell transplantation (GCT) in sexually competent f...