Carbonate apatite (CO3Ap), the form of apatite found in bone, has recently attracted attention. The purpose of the present study was to histologically evaluate the tissue/cellular response toward the low-crystalline CO3Ap fabricated using a dissolution-precipitation reaction with set gypsum as a precursor. When set gypsum was immersed in a 100°C 1 mol/L Na3PO4 aqueous solution for 24 h, the set...

Dopamine beta-hydroxylase (DBH) was purified from bovine adrenal medullae. Rabbit IgG raised against DBH inhibited its activity by 80%. In an immunoblot analysis, the IgG specifically recognized two subunits of DBH the 72 and 75 KD components. Chromogranin A (CGA) also was purified from bovine adrenal medullae, and rabbit IgG against CGA recognized this chromogranin A in the immunoblot analysis...

The immunoglobulin G (IgG) binding ZZ domain of protein A from Staphylococcus aureus was fused to the N terminus of the polyhydroxyalkanoate (PHA) synthase from Cupriavidus necator. The fusion protein was confirmed by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry and mediated formation of ZZ domain-displaying PHA granules in recombinant Escherichia coli. The IgG b...

Degenerating chondrocytes are often localized in the lower hypertrophic and calcifying zone of rat epiphyseal cartilage. The ability of these degenerating chondrocytes to synthesize and secrete sulfated proteoglycans was investigated using the light microscopic radiosulfate labeling method and the high-iron diamine-thiocarbohydrazide-silver proteinate (HID-TCH-SP) method for sulfated complex ca...

Neuromelanin is a complex polymer pigment found primarily in the dopaminergic neurons of human substantia nigra. Neuromelanin pigment is stored in granules including a protein matrix and lipid droplets. Neuromelanin granules are yet only partially characterised regarding their structure and function. To clarify the exact function of neuromelanin granules in humans, their enrichment and in-depth...

• Mathematical model for dissolution of a polydisperse collection binary granules. Full accounts radial variation in drug and porosity distributions. Reduced when initial concentration greatly exceeds solubility. Model single granule size validated against experimental data. Accurate mechanistic vitro models can deliver insight into release behaviour guide formulation development. Drug profiles...

The secretory end-pieces of the submandibular gland of rats during the first week of postnatal development are studied with regard to the fine structure of the secretion granules in these end-pieces. The terminal ends of the secretory ducts during this period consist of two types of cells; one cell is an acinar-type and the other is a duct-type found in the gland of adult rats. The secretion gr...