Mastitis is characterized by increased sodium and decreased potassium in milk, and the effect of this on bacterial growth was examined. Colonies of Staphylococcus aureus, Streptococcus uberis, and Escherichia coli were grown in tryptone broth containing 0−75 mM Na or 0−40 mM K. For S. aureus, the total viable count (TVC) growth rate decreased as Na increased from the normal level to that typica...

Staphylococcus aureus is the most common contagious pathogen associated with bovine subclinical mastitis. Current diagnosis of S. aureus mastitis is based on bacteriological culture of milk samples and somatic cell counts, which lack either sensitivity or specificity. Identification of milk proteins that contribute to host defense and their variable responses to pathogenic stimuli would enable ...

Mastitis is known as the most common disease syndrome in the dairy industry. Treating mastitic lactating animals with antibiotics is a veterinary practice to cure the disease. However, the antibiotic used may persist in the milk for a period of time depending on drug selected, dosage applied, route administered, body weight of the animal treated, etc. Antibiotic residues in milk are of great co...

OBJECTIVE To evaluate the elimination kinetics of chlorhexidine in milk when used as an intramammary infusion to stop lactation in cows. DESIGN Prospective study. ANIMALS 6 cows. PROCEDURE The study was performed in 2 phases. Three cows were studied in each phase. All cows were treated with chlorhexidine suspension by infusion into a mastitic mammary gland quarter after 2 milkings 24 hour...

The aims of this study were to isolation and identification of methicillin resistant Staphylococcus aureus (MRSA) from mastitic cow milk and nasal swabs of cows and related workers, investigation presence of Panton-Valentine leucocidin (PVL) genes and molecular characteristics of these isolates. One hundred forty five mastitic milk samples were obtained from of 56 cows and nasal swabs were coll...

Mycoplasma colonies and Mycoplasma cells in preparations from infected milk and lymph nodes were observed for their fluorescent qualities after treatment with acridine orange. Mycoplasma colonies fluoresced brilliant red or red-orange. When treated after exposure to ribonuclease, the colonies fluoresced lime-green. There was no fluorescence when both ribonucleic acid and deoxyribonucleic acid w...

The present study was designed to investigate the effect of mastitis on various components including fat percentage, protein, total solids, solid not fat, pH, specific gravity and lactose content in milk cross-bred (Holstein Friesian × Cholistani) Sahiwal cattle. A 376 samples (excluding blind nonfunctional teats) from 100 cows (50 50 sahiwal) were collected aseptically. Animals different ages,...