The publicly reported limited application of marker-assisted selection (MAS) in wheat breeding programmes to date is reviewed and contrasted with the current situation, in which it has increasingly become technically feasible to tag almost any gene with a microsatellite assay. Although this capability is starting to have an impact on the conduct of large breeding programmes, a much more profoun...

Rapid and accurate genome-wide marker detection is essential to the marker-assisted breeding and functional genomics studies. In this work, we developed an integrated software, AgroMarker Finder (AMF: http://erp.novelbio.com/AMF), for providing graphical user interface (GUI) to facilitate the recently developed restriction-site associated DNA (RAD) sequencing data analysis in rice. By applicati...

a study was conducted to identify ssr markers linked to leaf rust resistance genes lr24 and lr28 and to be used for marker-assisted selection (mas) to transfer both genes to a widely cultivated wheat variety mp 3299 under rainfed condition. f2 individuals of the cross mp 3299×nil pbw 343 were used for generating genotypic data employing closely linked scar markers s73719 and s421570 to lr24 and...

Allocating resources between population size and replication affects both genetic gain through phenotypic selection and quantitative trait loci detection power and effect estimation accuracy for marker-assisted selection (MAS). It is well known that because alleles are replicated across individuals in quantitative trait loci mapping and MAS, more resources should be allocated to increasing popu...

The incidence and prognostic relevance of bone marrow (BM) and leukapheresis (PBPC) tumor cell contamination (TCC) in breast cancer patients is still to be circumstantiated. We developed a new comprehensive gene expression panel to study cytokeratins (CK), maspin (MAS) and mammaglobin (MAM) as possible predictors of prognosis. Forty-eight patients undergoing high dose chemotherapy (HDCT) and PB...

pyramiding genes related to grain quality and resistance through marker assisted selection (mas) is an important approach in rice breeding programs. marker-assisted selection can be used for monitoring the presence or absence of these genes in breeding populations and can be combined with conventional breeding approaches. this study is a part of cultivar development program in iran through inte...

In our early experiments, the chloroform extract of D. crassirhizoma was demonstrated to contain the highest concentrations of total phloroglucinols among several extract fractions and possessed the most effective nematicidal activity. This study aimed to ascertain the ultrastructural changes in M. incognita after treatment with a D. crassirhizoma chloroform extract at 1 mg·mL⁻¹ for 24 h. It wa...

Marker aided selection (MAS) can be used for monitoring the presence or absence of genes in breeding populations and can be combined with conventional breeding approaches. In this study, crosses between two high yielding cultivars, Neda and Nemat with local aromatic varieties Sang tarom and Tarom deylamani were made followed by phenotypic selection for desirable individual plants in F2 populati...

The use of resistant cultivars is the most effective strategy for controlling coffee leaf rust caused by fungus Hemileia vastatrix. To assist development such cultivars, amplified fragment-length polymorphism (AFLP) markers linked to two loci resistance races I and II as well pathotype 001 H. vastatrix were converted sequence-characterized region (SCAR) cleaved polymorphic site (CAPS) markers. ...

There is a large gap between genomewide association studies (GWAS) and developing markers that can be used in marker-assisted selection (MAS) schemes for cultivar improvement. This study prototype using segregating single nucleotide polymorphisms (SNPs) panicle architecture grain shape traits identified by GWAS the Rice Diversity Panel-1 colocalized QTL regions revealed linkage mapping Estrela ...