Mycobacterium tuberculosis and M. bovis infect animals and humans. Their epidemiology in developed and developing countries differs, owing to differences in the implementation of preventive measures (World Health Organization, 1999). Identification and differentiation of these closely related mycobacterial species would help to determine the source, reservoirs of infection, and disease burden d...

a nested polymerase chain reaction (pcr) assay with two pairs of primers was applied for the detection of m. tuberculosis complex dna in specimens of 51 patients. of these, 31 clinically diagnosed patients (group i) recruited from july 1996 to june 1997 were given antituherculous chemotherapy from one to six months with standard daily regimen (isaniazid, 5 mg/kg; rifampin, 10 mg/kg; pyrazinamit...

A simplified polymerase chain reaction (PCR) assay was developed for fast and easy screening of mycoplasma mastitis in dairy cattle. Species of major mycoplasma strains [Mycoplasma (M.) bovis, M. arginini, M. bovigenitalium, M. californicum, M. bovirhinis, M. alkalescens and M. canadense] in cultured milk samples were detected by this simplified PCR-based method as well as a standard PCR techni...

Background: Production of β–lactamases by enterobacteriacea, especially Klebsiella pneumoniae, is one of the emerging health problems in the world. The purpose of this study was to assess the frequency of blaCTX-M15 gene in K. pneumoniae isolates and determine the molecular diversity of CTXM producing isolates. Methods: In...

BACKGROUND The diagnosis of pulmonary tuberculosis is still a major challenge. Using a polymerase chain reaction (PCR), one can detect Mycobacterium tuberculosis in clinical samples within a few hours. However, single gene targets may result in false negativity due to the absence of target DNA in some M. tuberculosis isolates. The objective of this study was to develop and evaluate a multiplex ...

The aim of this study was to compare conventional 16S rRNA gene PCR, real-time 16S rRNA gene PCR and real-time Mycoplasma genitalium adhesin protein (MgPa) gene PCR as detection methods for M. genitalium infection. The study also determined the prevalence of M. genitalium in male and female patients attending a sexually transmitted infections clinic in a rural area in the west of Sweden. First ...