A study was conducted to determine the requirement for lysine of a White Leghorn strain of hens with a body weight of approximately 1,600 g. Before starting the experiment, apparent fecal digestibility of amino acids of the basal diet was determined in an in vivo digestibility trial with six individually housed hens. The basal diet used was based on corn and soybean meal and contained 0.65% tot...

This study evaluated the dietary lysine requirement by measuring the plasma free lysine concentrations in rainbow trout, Oncorhynchus mykiss after dorsal aorta cannulation. A basal diet containing 36.6 % crude protein (29.6 % crystalline amino acids mixture, 5 % casein and 2 % gelatin) was formulated to one of the seven L-amino acid based diets containing graded levels of lysine (0.72, 1.12, 1....

BACKGROUND The current Dietary Reference Intake (DRI) recommendations for lysine requirements in children are based on a factorial estimate. OBJECTIVE The objective of the current study was to determine the lysine requirement in healthy school-age children by measuring the oxidation of l-[1-(13)C]phenylalanine to (13)CO(2) (F(13)CO(2)) in response to graded intakes of lysine. DESIGN Five he...

The aim of this study was to investigate whether the phases of the menstrual cycle affect lysine requirement in healthy adult females, as determined by the indicator amino acid oxidation (IAAO) method. Five healthy females with regular menstrual cycles were studied at seven graded levels of lysine intake, in random order, with an oral [13C]phenylalanine tracer protocol in both the follicular an...

A total of 163 sows were used in a study to determine the requirement for total sulfur amino acids (TSAA), relative to lysine, during lactation. All experimental diets were cornsoybean meal-based and formulated to contain 0.88% true ileal digestible (TID) lysine (0.97% total lysine). The experimental diets contained 0.37% L-lysine HCl, with other crystalline amino acids added to ensure that TSA...

Probably due to methodological problems the knowledge about the AA requirement for maintenance in pigs is rather scarce. In the present study an alternative experimental approach was applied and its underlying hypothesis was tested, whether protein retention decreases with body weight (BW), when daily lysine intake remains constant and acts as the limiting factor for protein retention, and whet...

and ADFI at week 4 (P = 0.02). During phase 2 (week 3 to 4), greater ADG (P = 0.05) and ADFI (P = 0.004) were observed in pigs consuming a diet containing DDGS (Treatments B and D) compared to pigs that did not receive DDGS in phase 1 (Treatments A and C); however, G:F ratio was not affected by DDGS. There was a lactose effect on ADG in week 3 (P = 0.05) and a trend of lactose effect on ADG dur...

The study was conducted to evaluate carcass yield and chemical composition of thigh and breast muscles of broilers fed three different lysine requirement levels: high lysine (+10% NRC), standard (NRC) and low lysine (-10% NRC) and two ways of expressing amino acids in feedstuffs (total or digestible amino acids). The results of this study showed that diet formulation based on digestible amino a...