• Differential Scanning Calorimetry analysis of complex media mixtures. Novel method for evaluating thermodynamic stability proteins in unpurified solutions. Protein structural effects purification on expressed proteins. ACE2-ACE2 inhibitor interactions analyzed media. Analysis SARS-CoV-2 RBD binding to ACE2 Screening ligands that can bind biologic products vitro expression systems typically re...

in this research, we investigated the cytotoxic mechanisms of cochlodinium polykrikoidescell lysate on isolated rat liver hepatocytes.this micro algae is responsible for a severe and widespread harmful algal bloom in the persian gulf and gulf of oman (2008-2009). isolated hepatocytes were obtained by collagenase perfusion of sprague-dawley rat liver.according to our results, incubation of algal...

a radioimmunoassay method for direct determination of eif-2 concentration in reticulocyte lysate has been developed. this assay method measures eif-2 concentratio ns in nanogram range. a value of 40 pmol eif-2 per ml of reticulocyte lysate was obtained.

Inflammatory bowel disease (IBD) is caused by dysregulation of colon mucosal immunity and mucosal epithelial barrier function. Recent studies have reported that lipoteichoic acid (LTA) from Lactobacillus plantarum K8 reduces excessive production of pro-inflammatory cytokine. In this study, we investigated the preventive effects of lysate of Lb. plantarum K8 in dextran sulfate sodium (DSS)-induc...

Cutaneous T-cell lymphoma (CTCL) is a lymphoproliferative skin disease with limited therapeutic options. Ten CTCL patients were treated with once-weekly intranodal injection of 1 x 106 mature monocyte-derived dendritic cells (DCs) pulsed with 100 microg/mL tumor lysate protein equivalent and keyhole limpet hemocyanin (50 microg/mL). Tumor-specific delayed-type hypersensitivity (DTH) reactions d...

The products of various strains of Escherichia coli (BL21, DH5alpha, HB101 and XL Blue) were investigated for antimycotic properties using pathogenic isolates of Aspergillus. Co-culture experiments revealed that E. coli strains exhibited variable activity against Aspergillus fumigatus. The lysates prepared from DH5alpha, HB101 and XL Blue strains of E. coli showed inhibitory activity against A....

Mannan synthetase activity in spheroplast lysates prepared from Saccharomyces cerevisiae was measured by following the incorporation of [14C]mannose from guanosine 5'-diphosphate-[14C]mannose into material precipitable with cold 0.3 M perchloric acid. When enzyme activity was assayed at high concentrations of spheroplast lysate protein (10 mg/ml) in the presence of 7.5 mM MnCl2, a severe inhibi...

PURPOSE This study was undertaken to investigate whether thioltransferase (TTase) exhibits dehydroascorbate (DHA) reductase activity in human lens epithelial cells. METHODS TTase was investigated for DHA reductase activity in vitro by the method of glutathione reductase-coupled spectrophotometric assay. DHA reductase activities of human lens epithelial (HLE-B3) cell lysate and TTase-depleted ...

Microalgae are a valuable source of enzymes and active ingredients due to their biochemical variability, health-beneficial properties, lower production cost viability on an industrial scale. In addition, the growing demand for sustainable products constitutes microalgae as viable resource development range innovative products. present work, proteolytic activity Arthrospira platensis lysate was ...