l-Phenylalanine ammonia-lyase (PAL) activity develops in excised bean axes after approximately 5 hours of incubation and reaches a maximum level after 14 hours of incubation. Light does not affect the development of activity, but puromycin, cycloheximide, actinomycin D, and 5-fluorouracil inhibit.During this period of incubation both d- and l-p-fluorophenylalanine stimulate fresh weight increas...

pseudomonas aeruginosa is an opportunistic pathogen that causes a variety of infections in compromised patients. the ability of pseudomonas aeruginosa to produce chronic infection is based in part on its ability to biosynthesis of biofilm, and alginate is the major polysaccharide in the synthesized biofilm. so alginate degradation is very essential in the dispersion of pseudomonas aeruginosa bi...

Phenylalanine ammonia-lyase (PAL) and peroxidase (POD) enzyme activities, total phenolics and pyruvic acid (PA) (as pungency indicator) were measured in onion bulb tissues (c.v. Rouge Amposta) during storage at 4 and 20 3C. After 2 wk, PAL activity increased at 4 3C, but decreased at 20 3C, and after 4 wk, PAL activity at 20 3C was higher than activity observed at 4 3C. Peroxidase activity was ...

Fresh asparagus (Asparagus officinalis L.) deteriorates rapidly after harvest. Early enzymatic changes that follow harvesting of asparagus are important factors contributing to postharvest deterioration. We held asparagus spears at 25°C for up to 5 days after harvest and examined changes in the activities of Acid invertase (AI), Sucrose Synthase (SS), Glutamine Synthetase (GS) and Phenylalanine...

Phenylketonuria (PKU) is an autosomal recessive genetic disorder in which mutations in the phenylalanine-4-hydroxylase (PAH) gene result in an inactive enzyme (PAH, EC 1.14.16.1). The effect is an inability to metabolize phenylalanine (Phe), translating into elevated levels of Phe in the bloodstream (hyperphenylalaninemia). If therapy is not implemented at birth, mental retardation can occur. P...

lignin is one of the major characteristics of plant secondary cell wall that provides structural rigidity for the cells and tissues and hydrophobicity to tracheary elements. internode tissues of aeluropus littoralis as a halophyte grass were sampled at different developmental stages (from the first to the fifth internodes ) and under different nacl concentrations. the influences of nacl and int...

Hoagland’s nutrient solution (HS) * was used as a tool to investigate whether or not the phyto­ chrome-mediated appearance of phenylalanine ammonia-lyase (PAL) * and ascorbate oxidase (AO) * in the mustard seedling cotyledons is simply a modulation of the appearance of these enzymes in the dark (basal level). HS was applied to the mustard (Sinapis alba L.) seedling at sowing instead of distille...

effects of pre-storage application of salicylic acid (sa) and storage periods were investigated on phenylalanine ammonia lyase (pal) activity, chilling index (ci) and quality of pomegranate (punica granatum) cv. malase saveh. the results showed that with increase in sa concentration up to 2mm, ci and ion leakage (il) were reduced while pal activity being increased. the lower il and ci vc the hi...

Objective(s): Pseudomonas aeruginosais a Gram-negative and aerobic rod bacterium that displays mucoid and non-mucoid phenotype. Mucoid strains secrete alginate, which is the main agent of biofilms in chronic P. aeruginosa infections, show high resistance to antibiotics; consequently, the biological disruption of mucoid P. aeruginosa biofilms is an attractive area of study for researchers. Algin...

Aromatic ammonia-lyases catalyze the non-oxidative ammonia elimination from aromatic Lamino-acids, producing α,β-unsaturated compounds. The members of this enzyme family are the phenylalanine ammonia-lyase (PAL, EC 4.3.1.24), (Hodgins, 1971) histidine ammonia-lyase (HAL, EC 4.3.1.3) (Givot et al., 1969; Wickner, 1969) and the tyrosine ammonia-lyase (TAL, EC 4.3.1.23) (Kyndt et al., 2002) cataly...