Effects of ATP and Mg2+ concentrations on bioluminescence reaction of luciferase (Photinus pyralis) were investigated by home-made luminometer. The Michaelis constants of the enzyme for ATP and Mg2+ obtained from the Lineweaver-Burk graph, were 61.9 mM ±3.3 mM and 251.6mM ± 39.0mM...

A chemiluminescent DNA probe (Accuprobe) assay developed by Gen Probe, Inc., for the rapid identification of Histoplasma capsulatum was evaluated and compared with the exoantigen test by using 162 coded cultures including Histoplasma capsulatum var. capsulatum, Histoplasma capsulatum var. duboisii, Histoplasma capsulatum var. farciminosum, Blastomyces dermatitidis, Coccidioides immitis, Paracoc...

BACKGROUND Firefly luciferase (Fluc) has routinely been used to quantitate and analyze gene expression in vitro by measuring the photons emitted after the addition of ATP and luciferin to a test sample. It is now possible to replace luminometer-based analysis of luciferase activity and measure luciferase activity delivered by viral vectors directly in live animals over time using digital imagin...

A multiplex single-nucleotide polymorphism (SNP) typing platform using 'bioluminometric assay coupled with terminator [2',3'-dideoxynucleoside triphosphates (ddNTPs)] incorporation' (named 'BATI' for short) was developed. All of the reactions are carried out in a single reaction chamber containing target DNAs, DNA polymerase, reagents necessary for converting PPi into ATP and reagents for lucif...

The present work was finalised to develop chemiluminescent assays more sensitive and easy to use than the analytical methods currently in use to detect traces of TNT (2,4,6-trinitrotoluene), TATP (Triacetone triperoxide) and HMTD (Hexamethylene triperoxide diamine). For TNT detection we developed an indirect competitive ELISA with chemiluminescent end-point (CL-ELISA) and a Lateral Flow ImmunoA...

Abstract For the Phase-2 CMS upgrade for High-Luminosity LHC, a luminosity uncertainty of 1% is targeted. To achieve this goal, measurements from multiple luminometers with orthogonal systematics are required. A standalone luminometer, Fast Beam Condition Monitor (FBCM) being designed online bunch-by-bunch measurement. The sampling time resolution ∼0.7 ns also enables measurement beam-induced b...

Common Western-blot imaging systems have previously been adapted to measure signals from luminescent microplate assays. This can be a cost saving measure as Western-blot imaging systems are common laboratory equipment and could substitute a dedicated luminometer if one is not otherwise available. One previously unrecognized limitation is that the signals captured by the cameras in these systems...

The aim of this note is to characterize briefly main components of theoretical error of the small angle Bhabha measurement at LEP and to discuss critically how solid these estimates really are, from todays perspective. We conclude that the existing theoretical error of the LEP luminometer process (small angle Bhabha) is rather solid, and we add some new discussion concerning the remaining uncer...

We interfaced a microcomputer on-line with a luminometer to acquire the light signal of chemiluminescent reactions from a photomultiplier and then compute significant parameters of light emission and kinetic "shape" indices. Using this system to study interferences from biological samples on the measurement of chemiluminescent reactions, we observed that such effects are usually associated with...