Despite their practical and commercial relevance, there are few reports on the kinetics of growth and production of Chinese hamster ovary (CHO) cells-the most frequently used host for the industrial production of therapeutic proteins. We characterize the kinetics of cell growth, substrate consumption, and product formation in naive and monoclonal antibody (mAb) producing recombinant CHO cells. ...

We report molecular-fluorescence enhancement via the blue-shifted plasmon-induced resonance energy transfer (PIRET) from single Au nanorods (AuNRs) to merocyanine (MC) dye molecules. The blue-shifted PIRET occurs when there is a proper spectral overlap between the scattering of AuNRs and the absorption of MC molecules. Along with the quenching of scattering from AuNRs, the blue-shifted PIRET en...

Skolpap, W., Scharer, J.M., Douglas, P.L. and Moo-Young, M. Optimal feed rate profiles for fed-batch culture in penicillin production Songklanakarin J. Sci. Technol., 2005, 27(5) : 1057-1064 The fed-batch optimization of penicillin productivity was applied as an example of optimization algorithm verification. The objective function of this problem was to optimize penicillin productivity by dete...

Background: Schizophyllum commune, is one of the important medicinal-fungi foods in the world. Due to its important constituents such as extracellular and intracellular polysaccharides, it is widely used in industry and medicine. One of the important polysaccharides of this fungus is chitin-glucan complex (CGC). The aim of this study was to investigate the growth of native fungus Schizophyllum ...

the potential of bulk starter fermentation strategy for production of a cost-effective and safe source of β-galactosidase was investigated. three different bulk starter media with different compositions were selected, and an industrial yogurt starter culture strain, l. bulgaricus dsm 20081 was cultivated in these media under ph-controlled conditions (ph=5.6) at 43°c. the media consisted of 1) b...

level: advanced M athematically modeling the kinetics of batch and continuous cultivation allows you to not only calculate and evaluate the effects of process parameters, but also to forecast those parameters and duration of cultivation to develop a costeffective production process. Mathematical modeling microorganism cultivation was intensively developed in the second half of the last century....

Optimization of process variables in the bioconversion of pretreated sugarcane bagasse using cellulase and Saccharomyces cerevisiae by Simultaneous Saccharification and Fermentation (SSF) was investigated in the present study. A 23 five level Central Composite Design (CCD) experiments with central and axial points were used to develop a statistical model for the optimization of process variable...

where 7/denotes the set of integers. Obviously F(D) has the structure of a field. An (n, h) convolutional code C of memory span m over F, is the F(D) row space m of a k × n polynomial matrix G(D) = ~i=0 Gi Di, where each G i (0 ~ i ~ m) is a k × n matrix overF. The rank of G(D) overF(D) must be k. Recently (Piret, 1978) binary (6, 2) codes were obtained as an application of convolutional operat...

Poly-β-hydroxybutyrate (PHB) is one of the most famous biopolymers that has various applications in production of biodegradable carriers. The most important strategy for enhancing efficiency in production process and reducing the price of PHB, is the accurate expression of kinetic model of products formation and parameters that are effective on it, such as Dry Cell Weight (DCW) and substrate co...