Cytotoxic effector lymphocytes were induced in cultures of mouse spleen or lymph node cells by lymphocytosis promoting factor (LPF). The LPF-activated cytotoxic cells: (a) were not generated unless proliferation occurred; (b) sedimented in the lighter density fraction of a bovine serum albumin gradient; (c) were large, blast-like cells; and (d) were lysed by Thy-1.2 antiserum plus complement an...

Escherichia coli O157:H7 causes food and waterborne enteric infections that can result in hemorrhagic colitis and life-threatening hemolytic uremic syndrome. Intimate adherence of the bacteria to intestinal epithelial cells is mediated by intimin, but E. coli O157:H7 also possess several other putative adhesins, including curli and two operons that encode long polar fimbriae (Lpf). To assess th...

Recent studies show that the metabolic effects of fructose may vary depending on the phase of its consumption along with the light/dark cycle. Here, we investigated the metabolic outcomes of fructose consumption by rats during either the light (LPF) or the dark (DPF) phases of the light/dark cycle. This experimental approach was combined with other interventions, including restriction of chow a...

The role of the filamentous hemagglutinin (FHA) and the lymphocytosis-promoting factor hemagglutinin (LPF) in pertussis pathogenesis and immunity is the subject of active investigation. To be certain of their role as protective antigens, the hemagglutinins must be pure and free of each other. This report describes procedures to separate and purify FHA and LPF from the culture supernatant of sta...

Lignin is a natural biopolymer with a complex three-dimensional network. It is the second most abundant natural polymer on earth. Commercially, lignin is largely obtained from the waste liquors of pulping and bioethanol productions. In this study, wheat straw alkali lignin (WSAL) was demethylated by using an in-situ generated Lewis acid under an optimized demethylation process. The demethylatio...

OBJECTIVE We have recently described a modification (Veil of Aphrodite) designed to preserve the lateral prostatic fascia (LPF) during robotic prostatectomy. Here, we histologically compare the Veil of Aphrodite technique (VT) and standard nerve-sparing technique (ST). METHODS Thirty-six consecutive prostatectomies performed by a single surgeon were processed by the whole-mount method. The ri...

The lymphocytosis-promoting factor of Bordetella pertussis is a potent mitogen for murine lymphocytes in vitro. The stimulatory response was not the result of specific antigen stimulation. Spleen and lymph node cells were responsive, whereas normal thymocytes were unresponsive. However, DNA replication was induced in cortisone-resistant thymocytes by lymphocytosis-promoting factor (LPF). Bone m...

The leukocytosis-promoting factor (LPF), purified from materials extracted from agar in which Bordetella pertussis was grown, caused leukocytosis and an increased sensitivity to histamine when as little as 0.04 mug of protein was given intravenously to mice. LPF was adsorbed onto erythrocytes, causing hemagglutination. As little as 0.03 mug of LPF protein agglutinated chicken erythrocytes in 0....

We give two optimal linear-time algorithms for computing the Longest Previous Factor (LPF) array corresponding to a string w. For any position i in w, LPF[i] gives the length of the longest factor of w starting at position i that occurs previously in w. Several properties and applications of LPF are investigated. They include computing the Lempel–Ziv factorization of a string and detecting all ...

The leukocytosis-promoting factor (LPF), purified from materials extracted from agar in which Bordetella pertussis was grown, caused leukocytosis and an increased sensitivity to histamine when as little as 0.04 Ag of protein was given intravenously to mice. LPF was adsorbed onto erythrocytes, causing hemagglutination. As little as 0.03 ,g of LPF protein agglutinated chicken erythrocytes in 0.05...