نتایج جستجو برای: loop mediated isothermal amplification method lamp
تعداد نتایج: 2179620 فیلتر نتایج به سال:
We report a one-pot, closed-vessel enzymatic assay that eliminates carryover contamination while preserving robust DNA amplification in loop-mediated isothermal amplification (LAMP), providing reliable and rapid detection of target DNA in contaminated samples.
همچنان عفونت با هرپس سیمپلکس ویروس ها، به عنوان یک مسأله ی جهانی که تهدیدکننده-ی سلامت افراد است، باقی مانده است. به طوری که مادران حامله بدون علامت می توانند توسط این ویروس باعث انسفالیت مرگبار در جنین خود گردند. بیماری های متعددی توسط تیپ یک این ویروس ایجاد می گردد که مهم ترین آن انسفالیت در نوزادان می باشد که هدف این مطالعه، توسعه ی یک تکنیک سریع، آسان و دارای حساسیت و ویژگی بالا برای تشخیص ...
The aim of this study was to produce a sensitive, simple and rapid diagnostic method for the detection of foot-and-mouth disease virus (FMDV) in suspected cases of FMD, a one-step single-tube method of reverse transcription loop-mediated isothermal polymerase amplification (RT-LAMP). A set of six common primers was designed to target the highly conserved region of the RNA polymerase 3Dpol gene ...
We developed a new loop-mediated isothermal amplification (LAMP) method to detect rrs, a 16S rRNA gene of pathogenic Leptospira spp. in urine. The method enables detection of two leptospiral cells per reaction mixture following boiling of urine specimens. The sensitivity of this method is higher than that of culture or of flaB nested PCR.
background: in human, sry (sex-determining region of the y chromosome) is the major gene for the testis-determining factor which is found in normal xy males and in the rare xx males, and it is absent in normal xx females and many xy females. there are several methods which can indicate a male genotype by the presence of the amplified product of sry gene. the aim of this study was to identify th...
Background and purpose: Salmonella is a group of Enterobacterias that cause infectious diseases in human and animals. Typhoid, bacteremia, enterocolitis and salmonellosis which are caused by this bacterium are considered major health concerns in developing countries, such as Iran. Quick, accurate, reliable and early diagnosis is vital to prevent Salmonella outbreak. Materials and methods: Ther...
It is difficult and time-consuming to distinguish Haemophilus influenzae from the genotypically similar Haemophilus parainfluenzae, which is a commensal of the human oral cavity. The novel nucleic acid amplification technique of loop-mediated isothermal amplification (LAMP), which amplifies DNA under isothermal conditions (63 degrees C) with high specificity, efficiency, and rapidity, was evalu...
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