Rhodom icrobium vannielii, Lipopolysaccharide, Medium-Dependent Growth Forms, Cell Types The compositions of lipopolysaccharides from the photoheterotrophic budding R hodom i­ crobium vannielii strains DSM 162, Rm5, E3 and 2/1 are reported. Common constituents of these lipopolysaccharides are glucose, mannose, glucosamine, glucuronic and galacturonic acids, 2-keto-3-deoxyoctonate (KDO) and the ...

Pyrogen reactions during cardiac catheterization are an alarming complication that frightens patients and baffles many physicans. This report describes a simple, reproducible, precise technique for the measurement of endotoxin-like activity on the inner and outer surfaces of catheters intended for intravascular insertion. This technique is useful in documenting the cause of patient reactions. Q...

Lipopolysaccharides from three gram-negative bacteria isolated from bale cotton and piggery air were analyzed for their chemical composition, and their pulmonary toxicity for guinea pigs, lethal toxicity for mice, and pyrogenicity for rabbits were measured. Lipopolysaccharides from Enterobacter agglomerans and Citrobacter freundii had closely related chemical compositions; both were pyrogenic f...

Lipopolysaccharide was prepared from the extracellular lipoglycopeptide produced by the lysine-requiring mutant Escherichia coli A.T.C.C. 12408 grown under lysine-limiting conditions. The lipid moiety, containing glucosamine phosphate and four fatty acids (lauric acid, myristic acid, beta-hydroxymyristic acid and palmitic acid) corresponded in composition to lipid A of known bacterial lipopolys...

1. A series of oligosaccharides was isolated from Salmonella milwaukee lipopolysaccharide by partial acid hydrolysis. 2. Structural studies on these oligosaccharides indicated that the O-specific side chain of this lipopolysaccharide has a repeating pentasaccharide unit that is probably alpha-d-galactosyl-(1-->3)-beta-d-galactosyl- (1-->3)-N-acetylgalactosaminyl-(1-->3)-N-acetyl- d-glucosaminyl...

The lipopolysaccharides and free lipid A from several strains of Rhodospirillaceae were assayed comparatively with those of Enterobacteriaceae in a number of biological tests. Free lipid A's from Rhodopseudomonas gelatinosa and Rhodospirillum tenue exhibited strong serological cross-reactions with each other and with free lipid A from Salmonella. Lipid A's from Rhodopseudomonas viridis and Rhod...

In a previous report (1) the isolation, toxicity and some chemical data concerning the lipopolysaccharide endotoxin of Neisseria gonorrhoeae was described. The present investigation includes the following: (a) an improved modification of lipopolysaccharide phosphoric acid ester preparation; (b) hydrolysis of lipopolysaccharide phosphoric acid ester and removal of fatty acids; (c) separation of ...

Several species of Salmonella, Citrobacter, and Arizona were examined for the presence of 3-amino sugars, which were isolated from lipopolysaccharide hydrolysates by cation exchange chromatography and identified by paper and cation exchange chromatography in several systems and by specific colorimetric procedures. 3-Amino-3,6-dideoxyglucose was identified in C. freundii 8090, C. freundii 869, S...