Bacterial endotoxin (lipopolysaccharide) depresses cardiovascular function; however, the mediators and signaling pathways that are responsible for the negative inotropic effects of lipopolysaccharide are not fully known. We used RNA interference to determine the relative role of tumor necrosis factor with respect to mediating the negative inotropic effects of lipopolysaccharide in isolated card...

AIMS The aim of the present study was to determine whether or not lipopolysaccharide from Actinobacillus actinomycetemcomitans could stimulate arginase activity in a murine macrophage cell line (RAW264.7 cells). METHODS RAW264.7 cells were treated with A. actinomycetemcomitans-lipopolysaccharide or lipopolysaccharide from Escherichia coli for 24 h. The effect of polymyxin B, l-norvaline, dl-n...

Wolucka, B. A., McNeil, M. R., de Hoffmann, E. and Chojnacki, T. and Brennan, P. J. (1994) J. Biol. Chem. 269,23328-23335 Lee, R.E., MikusovB, K., Brennan, P. J. and Besra, G. S. (1995) J. Am. Chem. SOC. 117, 11829-1 1832 Takayama, K. and Kilburn, J. 0. (1989) Antimicrob. Agents Chemother. 33, 1493-1499 MikuSovB, K., Slayden, R. A., Besra, G. S. and Brennan, P. J. (1995) Antimicrob. Agents Chem...

The mechanism of lipopolysaccharide (LPS) transport in Gram-negative bacteria from the inner membrane to the outer membrane is largely unknown. Here, we investigated the possibility that LPS transport proceeds via a soluble intermediate associated with a periplasmic chaperone analogous to the Lol-dependent transport mechanism of lipoproteins. Whereas newly synthesized lipoproteins could be rele...

Acidiphilium spp. are conspicuous dwellers of acidic, metal-rich environments. Indeed, they are among the most metal-resistant organisms; yet little is known about the mechanisms behind the metal tolerance in this genus. Acidiphilium sp. PM is an environmental isolate from Rio Tinto, an acidic, metal-laden river located in southwestern Spain. The characterization of its metal resistance reveale...

Streptococcus pneumoniae causes pneumonia by infecting the alveolar epithelium via binding to host receptors, such as platelet-activating factor receptor (PAFR). Although chronic periodontitis has been identified a risk factor, how periodontopathic bacteria cause is not known. We found that S. adhered PAFR expressed on A549 human epithelial cells stimulated Porphyromonas gingivalis culture supe...

The fine structure of lipopolysaccharide isolated from Thermoplasma acidophilum was examined by electron microscopy. Negative staining of the lipopolysaccharide revealed long, ribbon-like structures with some branching. The average width of the lipopolysaccharide ribbons was 5 nm. Treatment of the lipopolysaccharide with 0.5% sodium dodecyl sulfate resulted in the dissociation of the ribbon-lik...

objective(s): occludin is essential for proper assembly of tight junctions (tjs) which regulate paracellular endothelial permeability. omega-3 polyunsaturated fatty acids (ω-3 pufa) protect endothelial barrier function against injury. materials and methods: we examined anti-inflammatory effect of ω-3 pufa intake (30 mg/kg/day for 10 days) on expression and location of occludin in the aorta of a...

Lipopolysaccharide can induce myocardial cell injury, which has been widely used in the preparation of septic cardiomyopathy models. We herein aimed to evaluate protective effects sodium hydrosulfide against oxidative stress and endoplasmic reticulum on model explore whether protein kinase RNA-like kinase-eukaryotic initiation factor 2 alpha signaling pathways was involved. The sepsis induced e...