objective: reporter gene transfer to mammalian cells receives a great deal of attention due to its importance for molecular biology, embryology and developmental biology studies. among dna transfer technologies to eukaryotic cells, lipofection is known as the most widely used because of its easy handling procedure, low cell mortality and the natural pathway it undertakes. materials and methods:...

The targeted delivery of therapeutic genes into specific tissues, as well as the determination of the biological fate and potential toxicity of nanoparticles, remains a highly relevant challenge for gene-based therapies. Type 1 insulin-like growth factor receptor (IGF-1R), an important oncogene, is frequently over-expressed in lung cancer and mediates cancer cell proliferation as well as tumor ...

Dominant dystrophic epidermolysis bullosa (DDEB) is an inherited blistering skin disease caused by heterozygous mutations in COL7A1, which encodes type VII collagen (C7), the major component of anchoring fibrils that maintain integrity dermal-epidermal junction. Most COL7A1 DDEB are single nucleotide variants, resulting glycine substitutions within C7 triple helix. Functionally, such cause impa...

We describe a modified protocol for introducing yeast artificial chromosomes (YACs) into murine embryonic stem (ES) cells by lipofection. With a decreased DNA:cell ratio, increased concentration of condensing agents and altered culture conditions, this protocol reduces the requirement for YAC DNA to a few micrograms, improves the recovery of neomycin-resistant ES colonies and increases the yiel...

Recessive Dystrophic Epidermolysis Bullosa is an autosomal recessive disorder caused by loss of function mutations in COL7A1 causing mucocutaneous blistering and scarring, with considerable morbidity mortality. A few early phase gene therapy trials have been reported as potential therapeutic approaches. The targeted nature base editing well suited to restore functionality RDEB, if supported eff...