In recent times, biotechnological applications of microbial lipases in synthesis of many organic molecules have rapidly increased in non-aqueous media. Microbial lipases are the 'working horses' in biocatalysis and have been extensively studied when their exceptionally high stability in non-aqueous media has been discovered. Stability of lipases in organic solvents makes them commercially feasi...

The positional and fatty acid specificities of lipases from several different microorganisms have been examined by gas-liquid chromatographic analysis of the free fatty acids following action on mixed triglycerides of known composition. Three types of lipases were found. Lipases of the first type, obtained from several microorganisms, attacked primarily the 1-position of the triglyceride in a m...

The positional and fatty acid specificities of lipases from several different microorganisms have been examined by gas-liquid chromatographic analysis of the free fatty acids following action on mixed triglycerides of known composition. Three types of lipases were found. Lipases of the first type, obtained from several microorganisms, attacked primarily the 1-position of the triglyceride in a m...

Abstract Microbial lipases are most broadly used biocatalysts for environmental and industrial applications. Lipases catalyze the hydrolysis synthesis of long acyl chain esters have a characteristic folding pattern ?/? hydrolase with highly conserved catalytic triad (Serine, Aspartic/Glutamic acid Histidine). Mesophilic (optimal activity in neutral pH range, mesophilic temperature atmospheric p...

Lipases (3.1.1.3) are ubiquitous, biogenic enzymes which hydrolyses the triacylglycerols at water-oil interface. Many species of animals, plants, and microbes produces lipases. The organisms able to produce lipases could tolerate organic solvents, high temperature, is industrial interest. Apart from its natural aqueous catalysis it also performs synthetic reactions in non-aqueous conditions, ha...

pseudomonas aeruginosa ef2, atcc 9027 and atcc 19660 were grown in a continuous culture under tween 80 (polyoxyethylene sorbitan monooleate) limitation and optimum conditions (ph 6.5, 37°c at dilution rate of 0.05/h). culture supernatants were carefully removed and stored at -20°c. to purify the lipases, the culture supernatant was reduced in volume to approximately 10 ml by an ultrafiltration ...

Increasing interest in lipases has been observed at the end of the last century, due to their potential application, in (bio)degradation as well as in (bio)synthesis of glycerides. The advantages of the enzymatic hydrolysis over the chemical process consist of less energy requirements and higher quality of the obtained products. Beside this, lipases are also efficient in various reactions such ...