نتایج جستجو برای: linker b lentivirus plasmid after confirmation of cloning
تعداد نتایج: 21379645 فیلتر نتایج به سال:
Colistin resistance in Acinetobacter spp. is increasing, resulting potentially untreatable nosocomial infections. Plasmid-mediated colistin of particular concern due to its low fitness cost and potential transferability other bacterial strains species. This study investigated the mechanism a clinical nosocomialis isolate from Cape Town, South Africa. A colistin-resistant A. was identified blood...
due to the widespread applications of xylitol dehydrogenase, an enzyme used for the production of xylitol, the present study was designed for the cloning of xylitol dehydrogenase gene from glcunobacter oxydans dsm 2003. after extraction of genomic dna from this bacterium, xylitol dehydrogenase gene was replicated using polymerase chain reaction (pcr). the amplified product was entered into ptz5...
Background and Aim: Leishmania major p4 gene is normally expressed during amastigote form of the parasite and can be a good candidate for producing an effective vaccine. The aim of this study is to clone p4 gene in a suitable vector for further vaccine production studies .Materials and Methods: This is an experimental study. Leishmania promastigote was grown in N.N.N. medium and later in th...
BACKGROUND The HBV-X (HBX) protein is believed to contribute to the development of HCC. However, the molecular mechanisms involved in HBX- mediated hepatocarcinogenesis remain obscure. In this study, the effect of hepatitis B virus X gene and its protein product HBxAg on expression of p53 gene in Hep G2 cell line was investigated. METHODS Viral DNA extracted from HBV-positive serum and HBX ge...
abstract the present study was conducted to examine the effect of proficiency on students interaction while carrying out the grammar-based task(s) in collaboration. in particular, the study examines whether the level of proficiency affects the learners’ focus of attention to grammatical and lexical features. the study takes a further step and examines whether there is any difference in the use...
Abstract Background and purpose: E. coli O157:H7 is one of the intestinal pathogens that mediate serious damages in gastrointestinal track. The bacterium attachment in large intestine is mediated via some colonization factors mainly Type III secretion proteins that are involved in this process. Among these factors, EspA protein plays an important role in system foundation. The aim of this ...
To explore the possible mechanism of KiSS1 in control GnRH secretion participate in sexual development onset and normal reproduction regulation by investigate the changes of expression of KiSS1, GnRH in hypothalamus and LH, FSH, E2 in serum,by using RNA interference Mediated with Lentivirus-based Vectors, after Interfering expression of KiSS1. Constructed the eukaryotic expression plasmid of ra...
development of yeast artificial chromosome (y ac) vectors, molecular cloning of large segments of chromosomal dna, and their propagation in yeast cells has become feasible. overlapping y ac provides a route to the development of physical maps of entire mammalian chromosomes. a rapid method was developed to isolate and sequence termini of y ac inserts quickly. the y ac clone is digested with a r...
The complete mouse mitochondrial genome (16.3 kb) was directly cloned into a Bacillus subtilis genome (BGM) vector. Two DNA segments of 2.06 and 2.14 kb that flank the internal 12 kb of the mitochondrial DNA (mtDNA) were subcloned into an Escherichia coli plasmid. Subsequent integration of the plasmid at the cloning locus of the BGM vector yielded a derivative specific for the targeted cloning ...
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