Five nonradioactive probe assays were evaluated by using chemiluminescent and colormetric signals, along with two isotopic assays and cell culture, for the detection of poliovirus in concentrated water samples. In environmental samples, a 100% correlation existed between digoxigenin and single-stranded [32P]RNA probes. All probe assays detected more positive samples than the cell culture did.

BACKGROUND Hepatitis C virus (HCV) genotype 6 is common among patients from Southeast Asia and the surrounding regions, where HCV prevalence is also high. HCV genotype 6 has great genetic diversity and different response to antiviral therapy compared with the more commonly known genotype 1. AIM Our goal was to provide a systematic review of the current literature on the epidemiology, classifi...

Background: World health organization (WHO) recommends the use of line probe assays (LiPAs) for rapid drug susceptibility testing (DST). However, only a limited number of studies from Pakistan have documented the performance characteristics of line probe assays in testing multi-drug resistant (MDR) strains of Mycobacterium tuberculosis (MTB). Objectives: The objective of this work is to evaluat...

the objective of the study was to evaluate the cytotoxic effects of aqueous extract of cucumis melo fruit on human prostate cancer cell line (pc-3) using mtt and neutral red assays. the crude aqueous extract of c. melo was prepared by cold maceration method, filtered, concentrated and tested on pc-3 cell line. dose-dependent cytotoxic activities were exhibited on human prostate carcinoma pc-3 c...

BACKGROUND A new DNA line probe assay (Speed-oligo Mycobacteria, Vircell) has been launched for rapid differentiation of Mycobacterium spp. from cultures. Compared to other line-probe assays, Speed-oligo Mycobacteria covers a relatively limited spectrum of species but uses a simpler and faster dip-stick technique. The present multi-centre, multi-country study aimed at evaluating the utility and...