The iron-binding protein lactoferrin (Lf) present in blood is metabolized by the liver. Isolated rat hepatocytes vigorously endocytose bovine Lf via recycling Ca2(+)-dependent binding sites, but the uptake of iron from Lf by hepatocytes has not been examined. In this study, isolated rat hepatocytes were incubated with radiolabelled bovine Lf (125I-Lf, 59Fe-Lf or 125I-59Fe-Lf) at 37 degrees C, t...

Logical frameworks have enjoyed wide adoption as meta-languages for describing deductive systems. While the techniques for representing object languages in logical frameworks are relatively well understood, languages and techniques for metaprogramming with them are much less so. This paper presents work in progress on a programming language called Rogue-Sigma-Pi (RSP), in which general programs...

LF has been designed and successfully used as a meta-logical framework to represent and reason about object logics. Here we design a representation of the Isabelle logical framework in LF using the recently introduced module system for LF. The major novelty of our approach is that we can naturally represent the advanced Isabelle features of type classes and locales. Our representation of type c...

A 20-day treatment with LF15-0195, a deoxyspergualine analogue, induced allograft tolerance in a fully MHC-mismatched heart allograft model in the rat. Long-term allografts displayed minimal cell infiltration with no signs of chronic rejection. CD4+ spleen T cells from tolerant LF15-0195-treated recipients were able to suppress in vitro proliferation of allogeneic CD4+ T cells and to transfer t...

The standard methodology for representing deductive systems in LF identifies the object’s language’s context with the LF context. Consequently, any variable dealt with explicitly by any judgement or metatheorem must be last in the context. When the object language is dependently typed, this can pose a problem for establishing some metatheoretic results, since dependent hypotheses cannot be re-o...

We present a type system extending the dependent type theory LF, whose terms are more amenable to compact representation. This is achieved by carefully omitting certain subterms which are redundant in the sense that they can be recovered from the types of other subterms. This system is capable of omitting more redundant information than previous work in the same vein, because of its uniform tre...

Bacillus anthracis lethal toxin consists of the protective antigen (PA) and the metalloprotease lethal factor (LF). During cellular uptake PA forms pores in membranes of endosomes, and unfolded LF translocates through the pores into the cytosol. We have investigated whether host cell chaperones facilitate translocation of LF and the fusion protein LF(N)DTA. LF(N) mediates uptake of LF(N)DTA int...