Australia claimed freedom from Newcastle disease (ND) in 2001 following the successful eradication of virulent ND from infected farms. The virulent virus was shown to have been derived from a series of mutational changes of the F protein cleavage site in endemic lentogenic virus, with a pronounced increase in virulence from the precursor virus. However, the detection of further precursor ND vir...

Aim: Newcastle disease is still one of the major threats for poultry industry allover the world. Therefore, attempt was made in this study to use the SYBR Green I real-time PCR with melting curves analysis as for detection and differentiation of NDV strains in suspected infected birds. Materials and Methods: Two sets of primers were used to amplify matrix and fusion genes in samples collected f...

Relatively little is known about the distribution of avian paramyxoviruses (APMVs) among wild birds in Japan. Surveillance of APMV in migratory waterfowl was conducted in the San-in region of western Japan during winters of 2006 to 2012. A total of 16 avian paramyxoviruses consisting of 3 lentogenic Newcastle disease viruses (NDVs), 12 APMV-4 and 1 APMV-8 were isolated from 1,967 wild-bird feca...

Mesogenic vaccine strains of Newcastle disease virus (NDV) are widely used in many countries of Asia and Africa to control the Newcastle disease of poultry. In India, the mesogenic strain R2B was introduced in 1945; it protects adult chickens that have been preimmunized with a lentogenic vaccine virus and provides long-lasting immunity. In this article, we report the complete genome sequence of...

Background: In reverse genetics studies, minigenomes of negative sense RNA viruses are used to model the process virus replication and evaluate rescue system in course construction recombinant virus. current study, a minigenome (MG) for lentogenic D58 vaccine strain Avian orthoavulavirus 1 (AOAV-1) (Newcastle disease virus) was developed establish genetic this strain. Methods: The constructed b...