UNLABELLED L-Lactic acid, one of the most important chiral molecules and organic acids, is produced via pyruvate from carbohydrates in diverse microorganisms catalyzed by an NAD+-dependent L-lactate dehydrogenase. Naturally, Escherichia coli does not produce L-lactate in noticeable amounts, but can catabolize it via a dehydrogenation reaction mediated by an FMN-dependent L-lactate dehydrogenase...

Activity of D-lactate dehydrogenase (D-LDH) was shown not only in cell extracts from Megasphaera elsdenii grown on DL-lactate, but also in cell extracts from glucose-grown cells, although glucose-grown cells contained approximately half as much D-LDH as DL-lactate-grown cells. This indicates that the D-LDH of M. elsdenii is a constitutive enzyme. However, lactate racemase (LR) activity was pres...

To evaluate the presence of components of a putative Intracellular Lactate Shuttle (ILS) in neurons, we attempted to determine if monocarboxylate (e.g. lactate) transporter isoforms (MCT1 and -2) and lactate dehydrogenase (LDH) are coexpressed in neuronal mitochondria of rat brains. Immunohistochemical analyses of rat brain cross-sections showed MCT1, MCT2, and LDH to colocalize with the mitoch...

1. Differentiation of the anodic (LDHi and LDH2) and cathodic (LDHs) isoenzymes of lactate dehydrogenase in human sera can be accomplished by combining variable substrate (lactate or pyruvate) concentrations with specific inhibitors such as urea. 2. One technic useful in the clinical laboratory is based upon a colorimetric assay for total LDH activity in which the oxidation of lactate is couple...

abstract objective removing plasma from the platelet concentrate (pc) medium could be an effective way to increase the safety of this product. the goal of this study was to compare pc stored in plasma or in an additive solution (composol) with in vitro testing. materials and methods fifty-four single donor pcs were prepared from iranian blood transfusion organization (ibto). each pc unit was di...

PURPOSE To investigate the relationship between lactate dehydrogenase A (LDH-A) expression, lactate concentration, cell metabolism, and metastases in murine 4T1 breast tumors. EXPERIMENTAL DESIGN Inhibition of LDH-A expression and protein levels were achieved in a metastatic breast cancer cell line (4T1) using short hairpin RNA (shRNA) technology. The relationship between tumor LDH-A protein ...

Coronavirus is a new pandemic disease that began in Wuhan, China, and has since spread over the globe. The number of COVID-19 cases reported daily Iraq slowly increased. This study aims to investigate effect Covid-19 on normal range White Blood Cells (WBC), D-Dimer, Lactate Dehydrogenase (LDH). looked at 65 patients who had tested positive for SARS-CoV-2 using polymerase chain reaction analysis...

At present, there are few means to track symptomatic stages of CNS aging. Thus, although metabolic changes are implicated in mtDNA mutation-driven aging, the manifestations remain unclear. Here, we used normally aging and prematurely aging mtDNA mutator mice to establish a molecular link between mitochondrial dysfunction and abnormal metabolism in the aging process. Using proton magnetic resona...

Background & Objective: Neonatal jaundice is common reason for admission in neonatal units requiring further evaluation to prevent adverse neurodevelopmental outcome. Lactate dehydrogenase levels are high hemolysis. This study was aimed explore the diagnostic properties of Dehydrogenase (LDH)in hemolytic causes hyperbilirubinemia. Methods: In this observational cross sectional study, prospe...

Alterations in cellular metabolism are among the most consistent hallmarks of cancer. Herein we have investigated the relationship between increased aerobic lactate production and mitochondrial physiology in tumor cells. To diminish the ability of malignant cells to metabolize pyruvate to lactate, lactate dehydrogenase A (LDH-A) levels were knocked down by means of LDH-A short hairpin RNAs. Red...