We studied a patient with large granular lymphocyte (LGL) leukemia for evidence of human T-cell leukemia/lymphoma virus (HTLV) infection. Serum from this patient was positive for HTLV-I/ II antibodies by enzyme-linked immunosorbent assay (ELISA) and was confirmed positive in Western blot and radioimmunoprecipitation assays. Results of a synthetic peptide-based ELISA showed that the seropositivi...

lymphocyte leukemia. Semin Hematol. 2003;40:185-195. 3. Sivakumaran M, Richards SJ, Hunt K, et al. Patterns of CD16 and CD56 expression in persistent expansions of CD3 NKa lymphocytes are predictive for clonal T-cell receptor gene rearrangements. Br J Haematol. 1991;78:368377. 4. Lima M, Almeida J, Dos Anjos Teixeira M, et al. TCRalphabeta /CD4 large granular lymphocytosis: a new clonal T-cell ...

Clonal CD8(+)/T-cell receptor (TCR)αβ(+) T-cell large granular lymphocyte (T-LGL) proliferations constitute the most common subtype of T-LGL leukemia. Although the etiology of T-LGL leukemia is largely unknown, it has been hypothesized that chronic antigenic stimulation contributes to the pathogenesis of this disorder. In the present study, we explored the association between expanded TCR-Vβ an...

We studied a patient with large granular lymphocyte (LGL) leukemia for evidence of human T-cell leukemia/lymphoma virus (HTLV) infection. Serum from this patient was positive for HTLV-I/ II antibodies by enzyme-linked immunosorbent assay (ELISA) and was confirmed positive in Western blot and radioimmunoprecipitation assays. Results of a synthetic peptide-based ELISA showed that the seropositivi...

Twelve cases of T gamma LPD (lymphoproliferative disorders of Fc gamma receptor-bearing T cells) involving an expansion of large granular lymphocyte/natural killer (LGL/NK) cells were investigated for the expression of LGL/NK-associated markers and for T beta gene rearrangement. All the cases selected were classified as T gamma LPD on the basis of morphology, function, and phenotype of the circ...

A patient with a persistent CD3 negative large granular lymphocyte (LGL) proliferation with immunophenotypic and functional characteristics of natural killer cells is described. The LGL proliferation persisted and six years after diagnosis the patient developed a high grade B cell non-Hodgkin's lymphoma. Molecular studies demonstrated clonal B cell populations in the peripheral blood, distinct ...

Large granular lymphocyte (LGL) leukemia is characterized by a clonal expansion of either CD3 cytotoxic T or CD3 NK cells. Prominent clinical features of T-LGL leukemia include neutropenia, anemia and rheumatoid arthritis (RA). The terminal effector memory phenotype (CD3 /CD45RA /CD62L CD57 ) of T-LGL suggests a pivotal chronic antigen-driven immune response. LGL survival is then promoted by pl...

A clinical entitity of large granular lymphocyte (LGL) leukemia was established based on the observation of clonality and the demonstration of tissue invasion by LGL of marrow, spleen, and liver.' The clonal expansion of LGL can be divided into two major lineages: CD3+ and CD3(natural killer: NK); however, it is still difficult to prove clonality in CD3NK LGL proliferation because CD3cells do n...

T cell large granular lymphocyte (T-LGL) leukemia features a clonal expansion of antigen-primed, competent, cytotoxic T lymphocytes (CTL). To systematically understand signaling components that determine the survival of CTL in T-LGL leukemia, we constructed a T-LGL survival signaling network by integrating the signaling pathways involved in normal CTL activation and the known deregulations of s...

A clinical entitity of large granular lymphocyte (LGL) leukemia was established based on the observation of clonality and the demonstration of tissue invasion by LGL of marrow, spleen, and liver.' The clonal expansion of LGL can be divided into two major lineages: CD3+ and CD3(natural killer: NK); however, it is still difficult to prove clonality in CD3NK LGL proliferation because CD3cells do n...