The Bacillus subtilis yusCBA operon, which encodes an ABC-type transporter, contains an S-box motif in its promoter region. We showed that the expression of these genes is repressed via the S-box system when methionine is available. The YusCB proteins are involved in the transport of both d- and l-methionine but also methionine sulfoxide. A yusCB mutant is unable to grow in the presence of 5 mi...

It has been shown previously (1,2) that the synthesis of CO-NH linkages by papain as well as by other proteinases is characterized by extreme stereochemical specificity. Thus, upon the addition of cysteine-activated papain to a mixture of carbobenzoxy-nn-glutamic acid and aniline, there is formed carbobenzoxy-L-glutamic acid anilide, which separates in crystalline form. The carbobenzoxy-n-gluta...

Methionine has an almost unique stimulatory effect on biosynthesis of cephalosporins (by Cephalosporium acremonium). No other sulfur-containing compound tested, except dl-methionine-dl-sulfoxide, replaced methionine. dl-Methionine stimulated the synthesis of cephalosporins when added after the growth phase. The utilization of inorganic sulfate was repressed by methionine. Experiments with l-met...

Suspensions of single cells were prepared from normal adult rat liver by perfusion of liver with and shaking in buffered solutions of 0.1% hyaluronidase and 0.05 % collagenase. L-[lJ4C]Leucine and L-[lJ4C]methionine incorporated into protein could be measured by precipitating the cells on filter paper disks without prior homogenization. No incorporation of amino acids into protein was observed ...

The Cover Feature shows a metal nanoparticle modified with the amino acid L-methionine and adsorption of enzyme acetylcholinesterase (AChE) on methionine-capped surface. Methionine-capped Ag Au nanoparticles (NPs) were tested as inhibitors AChE, they showed moderate inhibitory potential toward AChE. NPs are much larger than molecules cannot enter active site gorge, so inhibition occurs by block...

Enzymatic activities that could be involved in methanethiol generation in five cheese-ripening bacteria were assayed, and the major sulfur compounds produced were identified. L-Methionine and alpha-keto-gamma-methyl-thio-butyric acid demethiolating activities were detected in whole cells and cell extracts (CFEs) of all the bacteria tested. No L-methionine deaminase activity could be detected in...

In immature rib segments prepared from morning-glory (Ipomoea tricolor) flower buds, the major soluble metabolite formed from tracer amounts of l-methionine-U-(14)C was S-methylmethionine (SMM). In segments of senescing ribs, (14)C was progressively lost from SMM and appeared in free methionine. Immature segments contained about 4 nmoles of free methionine and about 16 nmoles of SMM per 30 segm...

The sulfur requirements of tobacco (Nicotiana tabacum L. var. Xanthi) XD cells grown in chemically defined liquid media can be satisfied by sulfate, thiosulfate, l-cyst(e)ine, l-methionine or glutathione, and somewhat less effectively by d-cyst (e) ine, d-methionine or dl-homocyst (e)ine. Sulfate uptake is inhibited after a 2 hr lag by l-cyst (e)ine, l-methionine, l-homocyst(e)ine or l-isoleuci...

Eight ruminally cannulated steers (294 kg, ADG = 1.3 kg/d) were used in a N retention study (8 x 8 latin-square design) to evaluate sulfur-containing (S) amino acid (AA) requirements for growth. Treatments were abomasal infusions of seven levels of L-methionine (0, 3, 6, 9, 12, 15 and 18 g/d) and one level of DL-methionine (6 g/d). All steers were fed a semipurified diet based on ammoniated cor...