The causal agent of Huanglongbing disease, 'Candidatus Liberibacter asiaticus', is a non-culturable, gram negative, phloem-limited α-proteobacterium. Current methods to control the spread of this disease are still limited to the removal and destruction of infected trees. In this study, we identified and characterized a regulon from 'Ca. L. asiaticus' involved in cell wall remodeling, that conta...

Sinorhizobium meliloti strain 1021, a nitrogen-fixing, root-nodulating bacterial microsymbiont of alfalfa, has a 3.5 Mbp circular chromosome and two megaplasmids including 1.3 Mbp pSymA carrying nonessential 'accessory' genes for nitrogen fixation (nif), nodulation and host specificity (nod). A related bacterium, psyllid-vectored 'Ca. Liberibacter asiaticus,' is an obligate phytopathogen with a...

Single-step and nested polymerase chain reactions (PCR) were used to determine the presence of Candidatus Liberibacter asiaticus, the phloem-limited bacterial pathogen of Huanglongbing (HLB), in leaves of wampee [Clausena lansium (Lour.) Skeels], lemon [Citrus limon (L.) Burm.] and several other citrus species. Specific PCR products were obtained when single-step PCR and nested PCR were used to...

CLIBASIA_01510, PrbP, is a predicted RNA polymerase binding protein in Liberibacter asiaticus. PrbP was found to regulate expression of a small subset of ribosomal genes through interactions with the β-subunit of the RNA polymerase and a short, specific sequence on the promoter region. Molecular screening assays were performed to identify small molecules that interact with PrbP in vitro. Chemic...

Citrus production in Florida declined steadily because of Huanglongbing (i.e., HLB, citrus greening) caused by the bacteria Candidatus Liberibacter asiaticus. The objectives study were to determine whether leaf tissue nutrient concentration, tree growth, fruit yield, and juice content improved with split applications essential nutrients on selected rootstocks sandy soils. treatments arranged a ...

Munyaneza, J. E., Sengoda, V. G., Buchman, J. L., and Fisher, T. W. 2012. Effects of temperature on ‘Candidatus Liberibacter solanacearum’ and zebra chip potato disease symptom development. Plant Dis. 96:18-23. Temperature has been shown to have a significant effect on development of liberibacter species associated with citrus Huanglongbing disease. ‘Candidatus Liberibacter africanus’ and ‘Ca. ...

Huanglongbing (HLB) is one of the most devastating diseases of citrus worldwide, and is caused by a phloem-limited fastidious prokaryotic alpha-proteobacterium that is yet to be cultured. In this study, a combination of traditional polymerase chain reaction (PCR) and real-time PCR targeting the putative DNA polymerase and 16S rDNA sequence of 'Candidatus Liberibacter asiaticus,' respectively, w...

‘Candidatus Liberibacter asiaticus’ is an insect-transmitted, phloem-restricted ?-proteobacterium associated with huanglongbing. Here, we provide the whole genome sequence of ‘Ca. L. strain ReuSP1 from its insect vector Diaphorina citri (Hemiptera: Liviidae) collected in La Réunion. The composed 1,230,064 bp and has a 36.5% G+C content. This study reports first Réunion, which will add to resour...