This two-site immunoradiometric assay for human parathyrin-related protein 1-86 (PTHRP1-86) in plasma uses a mouse monoclonal antibody to PTHRP1-34 coupled to cellulose particles for immunoextraction of N-terminal immunoreactivity, and a rabbit antiserum to PTHRP37-67 that is indirectly labeled with 125I-labeled PTHRP37-67 for quantifying the bound analyte. The detection limit of the assay is 0...

Fragments of Lactococcus lactis subsp. lactis NP45 chromosomal DNA provided promoter activity in Escherichia coli when cloned into the promoter probe vector pGKV210. Only 13% of these recombinant plasmids promoted detectable cat-86 activity when transferred to L. lactis, i.e., expressed chloramphenicol resistance. In these promoter-containing versions of pGKV210, the cat-86 gene specifies chlor...

It is therefore very important to optimise the care of diabetic patients at primary, secondary and tertiary care level. Numerous clinical practice guidelines for management of diabetes have been compiled and circulated to health care workers, but despite this the level of diabetes care is still not ideal because of suboptimal implementation strategies. Guideline implementation problems are a si...

86 NATURE | VOL 402 | 4 NOVEMBER 1999 | www.nature.com Calculation of correlated mRNA expression Results of 97 individual publicly available DNA chip yeast mRNA expression data sets were encoded as a string of 97 numbers associated with each yeast open reading frame (ORF) describing how the mRNA of that ORF changed levels during normal growth, glucose starvation, sporulation and expression of m...

0196-8904/$ see front matter 2013 Elsevier Ltd. All rights reserved. http://dx.doi.org/10.1016/j.enconman.2013.11.007 ⇑ Corresponding author. Tel.: +86 755 88018525; fax: +86 755 88018536. E-mail addresses: [email protected] (L. Jian), [email protected] (X. Zhu), [email protected] (Z. Shao), [email protected] (S. Niu), [email protected] (C.C. Chan). Linni Jian a,⇑, Xinyu Zhu , Zi...