Oxidation of ferrous iron by Thiobacillus ferrooxidans SM-4 was inhibited competitively by increasing concentrations of ferric iron or cells. A kinetic analysis showed that binding of one inhibitor did not exclude binding of the other and led to synergistic inhibition by the two inhibitors. Binding of one inhibitor, however, was affected by the other inhibitor, and the apparent inhibition const...

Characterizing macromolecular kinetics from molecular dynamics (MD) simulations requires a distance metric that can distinguish slowly interconverting states. Here, we build upon diffusion map theory and define a kinetic distance metric for irreducible Markov processes that quantifies how slowly molecular conformations interconvert. The kinetic distance can be computed given a model that approx...

5-Gluconolactone is a potent inhibitor of rabbit muscle phosphorylase (I in the presence of saturating AMP. Kinetic analysis leads to the conclusion that the inhibitor binds most strongly to the enzyme-glycogen-Pi complex (KAPI = 0.025 mm), but weaker binding is also observed with the enzymeglycogen-ar-o-glucopyranose l-phosphate complex, the enzyme-glycogen complex, the enzyme-Pi complex, and ...

2-Benzyl-3-hydroxybutanoic acid(1) was prepared starting with acetoacitic ester following benzylation, reduction using sodium dithionite and hydrolysis under alkaline condition. The inhibitory kinetic evaluation showed that 1(Ki =107μM) is potent than 2-benzyl-3-hydroxypropanoic acid(Ki =610μM) as inhibitor for carboxypeptidase A, suggesting that introducing of a methyl group at the β-position ...

Both equilibrium and nonequilibrium factors influence the efficacy of pharmaceutical agents that target intermediate states of biochemical reactions. We explored the intermediate state inhibition of gp41, part of the HIV-1 envelope glycoprotein complex (Env) that promotes viral entry through membrane fusion. This process involves a series of gp41 conformational changes coordinated by Env intera...

1. The supernatant obtained by centrifugation of a rat liver homogenate at 100000g for 1h contained a heat-labile macromolecular inhibitor of the thrombin-fibrinogen reaction. 2. The inhibitor was purified to electrophoretic homogeneity by repeated preparative polyacrylamide disc electrophoresis. Inhibition was observed with purified inhibitor equivalent to about 1mug of protein/ml. 3. The inhi...