1. Alkaline-earth-metal cations at low concentrations form soluble complexes with bovine caseins. The relative order of binding capacities is: Mg(2+)>Ca(2+)>Ba(2+)>Sr(2+). 2. The cations interact with both free ionized carboxyl groups of aspartic acid and glutamic acid and with monoester phosphate groups covalently bound to serine and threonine; at low concentrations of the cations interactions...

Abstract Genetically engineered chymosin from the tree shrew ( Tupaia belangeri chinensis ) has been obtained and partially characterized for first time. The target enzyme was produced in Escherichia coli , strain BL21(DE3). It shown that recombinant coagulates cow milk Bos taurus ). total specific milk-clotting activity of 0.7–5.3 IMCU/mL 8.8–16.6 IMCU/mg. nonspecific proteolytic relation to b...

Kappa-casein, as a major protein component in mammalian milk, plays an essential role in formation and stabilization milk micelles and preventing them from aggregating and therefore, helping to keep calcium phosphate in solution and transfer of calcium and phosphors from animal milk to consumers. Therefore, the objective of the current study was to investigate genetic and phylogenetic analysis ...